验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, A549 cells, Jurkat cells, HEK-293 cells, 4T1 cells, HSC-T6 cells, NIH/3T3 cells, pig brain tissue, rat brain tissue, mouse brain tissue |
Positive IHC detected in | human liver tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 7 publications below |
产品信息
67690-1-Ig targets NDUFB8 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human, mouse, rat, pig, rabbit |
文献引用反应性 | human, mouse, rat |
免疫原 | NDUFB8 fusion protein Ag6569 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa |
别名 | Complex I-ASHI, CI-ASHI, CIASHI, CI ASHI, ASHI |
计算分子量 | 22 kDa |
观测分子量 | 18-22 kDa |
GenBank蛋白编号 | BC000466 |
基因名称 | NDUFB8 |
Gene ID (NCBI) | 4714 |
RRID | AB_2882883 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | O95169 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for NDUFB8 antibody 67690-1-Ig | Download protocol |
IHC protocol for NDUFB8 antibody 67690-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Front Aging Neurosci Hippocampus-Based Mitochondrial Respiratory Function Decline Is Responsible for Perioperative Neurocognitive Disorders. | ||
Phytomedicine Zhen Wu decoction represses renal fibrosis by invigorating tubular NRF2 and TFAM to fuel mitochondrial bioenergetics | ||
Phytother Res Rutin-activated adipose tissue thermogenesis is correlated with increased intestinal short-chain fatty acid levels. | ||
Front Endocrinol (Lausanne) Androgen Excess Induced Mitochondrial Abnormality in Ovarian Granulosa Cells in a Rat Model of Polycystic Ovary Syndrome. | ||
Sci Total Environ Fluoride impairs mitochondrial translation by targeting miR-221-3p/c-Fos/RMND1 axis contributing to neurodevelopment defects | ||
Chin J Nat Med Mulberry leaf flavonoids activate BAT and induce browning of WAT to improve type 2 diabetes via regulating the AMPK/SIRT1/PGC-1α signaling pathway |