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NDUFB8 Monoclonal antibody

NDUFB8 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67690-1-Ig

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CloneNo. 3B12C6

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, ELISA

Complex I-ASHI, CI-ASHI, CIASHI, CI ASHI, ASHI

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, A549 cells, Jurkat cells, HEK-293 cells, 4T1 cells, HSC-T6 cells, NIH/3T3 cells, pig brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman liver tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 7 publications below

产品信息

67690-1-Ig targets NDUFB8 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat, pig, rabbit
Cited Reactivityhuman, mouse, rat
Immunogen NDUFB8 fusion protein Ag6569 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa
Synonyms Complex I-ASHI, CI-ASHI, CIASHI, CI ASHI, ASHI
Calculated Molecular Weight 22 kDa
Observed Molecular Weight18-22 kDa
GenBank Accession NumberBC000466
Gene Symbol NDUFB8
Gene ID (NCBI) 4714
RRIDAB_2882883
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95169
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NDUFB8 antibody 67690-1-IgDownload protocol
IHC protocol for NDUFB8 antibody 67690-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Front Aging Neurosci

Hippocampus-Based Mitochondrial Respiratory Function Decline Is Responsible for Perioperative Neurocognitive Disorders.

Authors - Keqiang He
mouseWB

Phytomedicine

Zhen Wu decoction represses renal fibrosis by invigorating tubular NRF2 and TFAM to fuel mitochondrial bioenergetics

Authors - Min Zheng
mouseWB

Phytother Res

Rutin-activated adipose tissue thermogenesis is correlated with increased intestinal short-chain fatty acid levels.

Authors - Long Cheng
ratWB

Front Endocrinol (Lausanne)

Androgen Excess Induced Mitochondrial Abnormality in Ovarian Granulosa Cells in a Rat Model of Polycystic Ovary Syndrome.

Authors - Linyi Song
ratWB

Sci Total Environ

Fluoride impairs mitochondrial translation by targeting miR-221-3p/c-Fos/RMND1 axis contributing to neurodevelopment defects

Authors - Dongjie Li
mouseWB

Chin J Nat Med

Mulberry leaf flavonoids activate BAT and induce browning of WAT to improve type 2 diabetes via regulating the AMPK/SIRT1/PGC-1α signaling pathway

Authors - Long Cheng
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