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NDUFB8 Recombinant antibody

NDUFB8 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

230510B10

Cat no : 83216-3-RR

Print datasheet

Synonyms

Complex I-ASHI, CI-ASHI, CIASHI, CI ASHI, ASHI

验证数据展示

  • WB analysis using 83216-3-RR

    WB analysis using 83216-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83216-3-RR (NDUFB8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 83216-3-RR

    IP experiment of HepG2 using 83216-3-RR

    IP result of anti-NDUFB8 (IP:83216-3-RR, 4ug; Detection:83216-3-RR 1:3000) with HepG2 cells lysate 1470 ug.

  • IF Staining of HepG2 using 83216-3-RR

    IF Staining of HepG2 using 83216-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFB8 antibody (83216-3-RR, Clone: 230510B10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of HepG2 using 83216-3-RR

    IF Staining of HepG2 using 83216-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFB8 antibody (83216-3-RR, Clone: 230510B10 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 83216-3-RR

    FC experiment of HeLa using 83216-3-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug NDUFB8 Recombinant antibody (83216-3-RR, Clone:230510B10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83216-3-RR

    Affinity and Kinetic Characterization of 83216-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83216-3-RR against Human NDUFB8 were performed. The affinity constant is 3.26 nM.

经过测试的应用

Positive WB detected inHepG2 cells, A549 cells, mouse liver tissue, mouse brain tissue, rat brain tissue, HeLa cells, Jurkat cells
Positive IP detected inHepG2 cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83216-3-RR is NDUFB8 Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, FC (Intra), IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen NDUFB8 fusion protein Ag6495 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa
Synonyms Complex I-ASHI, CI-ASHI, CIASHI, CI ASHI, ASHI
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 19-22 kDa
GenBank Accession NumberBC000466
Gene Symbol NDUFB8
Gene ID (NCBI) 4714
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO95169
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NDUFB8 antibody 83216-3-RRDownload protocol
IF protocol for NDUFB8 antibody 83216-3-RRDownload protocol
IP protocol for NDUFB8 antibody 83216-3-RRDownload protocol
FC protocol for NDUFB8 antibody 83216-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols