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验证数据展示

  • WB analysis using 83216-3-RR

    WB analysis using 83216-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83216-3-RR (NDUFB8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 83216-3-RR

    IP experiment of HepG2 using 83216-3-RR

    IP result of anti-NDUFB8 (IP:83216-3-RR, 4ug; Detection:83216-3-RR 1:3000) with HepG2 cells lysate 1470 ug.

  • IHC staining of human stomach cancer using 83216-3-RR

    IHC staining of human stomach cancer using 83216-3-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83216-3-RR (NDUFB8 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

  • IHC staining of human intrahepatic cholangiocarcinoma using 83216-3-RR

    IHC staining of human intrahepatic cholangiocarcinoma using 83216-3-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83216-3-RR (NDUFB8 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 83216-3-RR

    IF Staining of HepG2 using 83216-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFB8 antibody (83216-3-RR, Clone: 230510B10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of HepG2 using 83216-3-RR

    IF Staining of HepG2 using 83216-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFB8 antibody (83216-3-RR, Clone: 230510B10 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 83216-3-RR

    FC experiment of HeLa using 83216-3-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug NDUFB8 Recombinant antibody (83216-3-RR, Clone:230510B10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83216-3-RR

    Affinity and Kinetic Characterization of 83216-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83216-3-RR against Human NDUFB8 were performed. The affinity constant is 3.26 nM.

NDUFB8 Recombinant antibody

NDUFB8 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 83216-3-RR

产品说明书

CloneNo. 230510B10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

别名

Complex I-ASHI, CI-ASHI, CIASHI, CI ASHI, ASHI

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, A549 cells, mouse liver tissue, mouse brain tissue, rat brain tissue, HeLa cells, Jurkat cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman stomach cancer tissue, human intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83216-3-RR targets NDUFB8 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NDUFB8 fusion protein Ag6495 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa
别名 Complex I-ASHI, CI-ASHI, CIASHI, CI ASHI, ASHI
计算分子量 22 kDa
观测分子量 19-22 kDa
GenBank蛋白编号BC000466
基因名称 NDUFB8
Gene ID (NCBI) 4714
RRIDAB_3670901
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO95169
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NDUFB8 antibody 83216-3-RRDownload protocol
IHC protocol for NDUFB8 antibody 83216-3-RRDownload protocol
IF protocol for NDUFB8 antibody 83216-3-RRDownload protocol
IP protocol for NDUFB8 antibody 83216-3-RRDownload protocol
FC protocol for NDUFB8 antibody 83216-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols