NDUFB8 Recombinant antibody

NDUFB8 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

230510B10

Cat no : 83216-3-RR

Print datasheet

Synonyms

Complex I-ASHI, CI-ASHI, CIASHI, CI ASHI, ASHI



经过测试的应用

Positive WB detected inHepG2 cells, A549 cells, mouse liver tissue, mouse brain tissue, rat brain tissue, HeLa cells, Jurkat cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman stomach cancer tissue, human intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83216-3-RR targets NDUFB8 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen NDUFB8 fusion protein Ag6495 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa
Synonyms Complex I-ASHI, CI-ASHI, CIASHI, CI ASHI, ASHI
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 19-22 kDa
GenBank Accession NumberBC000466
Gene Symbol NDUFB8
Gene ID (NCBI) 4714
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO95169
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NDUFB8 antibody 83216-3-RRDownload protocol
IHC protocol for NDUFB8 antibody 83216-3-RRDownload protocol
IF protocol for NDUFB8 antibody 83216-3-RRDownload protocol
IP protocol for NDUFB8 antibody 83216-3-RRDownload protocol
FC protocol for NDUFB8 antibody 83216-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols