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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83216-4-RR (same clone as 83216-4-PBS)

    WB analysis using 83216-4-RR (same clone as 83216-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83216-4-RR (NDUFB8 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83216-4-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 83216-4-RR (same clone as 83216-4-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 83216-4-RR (same clone as 83216-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83216-4-RR (NDUFB8 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83216-4-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83216-4-RR (same clone as 83216-4-PBS)

    IF Staining of HepG2 using 83216-4-RR (same clone as 83216-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFB8 antibody (83216-4-RR, Clone: 230510H1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83216-4-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83216-4-RR (same clone as 83216-4-PBS)

    FC experiment of HeLa using 83216-4-RR (same clone as 83216-4-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug NDUFB8 Recombinant antibody (83216-4-RR, Clone:230510H1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83216-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83216-4-RR

    Affinity and Kinetic Characterization of 83216-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83216-4-RR against Human NDUFB8 were performed. The affinity constant is 5.00 nM.

NDUFB8 Recombinant antibody, PBS Only

NDUFB8 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83216-4-PBS

产品说明书

CloneNo. 230510H1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

Complex I-ASHI, CI-ASHI, CIASHI, CI ASHI, ASHI

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83216-4-PBS targets NDUFB8 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NDUFB8 fusion protein Ag6495 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa
别名 Complex I-ASHI, CI-ASHI, CIASHI, CI ASHI, ASHI
计算分子量 22 kDa
观测分子量 19-22 kDa
GenBank蛋白编号BC000466
基因名称 NDUFB8
Gene ID (NCBI) 4714
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO95169
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C