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NDUFB9 Polyclonal antibody

NDUFB9 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15572-1-AP

Print datasheet

Synonyms

B22, CI B22, Complex I B22, LYR motif containing protein 3, LYRM3, NDUFB9, UQOR22

验证数据展示

  • WB analysis using 15572-1-AP

    WB analysis using 15572-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15572-1-AP (NDUFB9 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 15572-1-AP

    IHC staining of human liver using 15572-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15572-1-AP (NDUFB9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, mouse pancreas tissue, rat pancreas tissue
Positive IHC detected inhuman liver tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

15572-1-AP targets NDUFB9 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen NDUFB9 fusion protein Ag7916 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa
Synonyms B22, CI B22, Complex I B22, LYR motif containing protein 3, LYRM3, NDUFB9, UQOR22
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC007672
Gene Symbol NDUFB9
Gene ID (NCBI) 4715
RRIDAB_2267110
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6M9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NDUFB9 antibody 15572-1-APDownload protocol
IHC protocol for NDUFB9 antibody 15572-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Commun

ZHX2 emerges as a negative regulator of mitochondrial oxidative phosphorylation during acute liver injury

Authors - Yankun Zhang
View Article
mouseWB

Circulation

Nuclear Receptor NR1D1 Regulates Abdominal Aortic Aneurysm Development by Targeting the Mitochondrial Tricarboxylic Acid Cycle Enzyme Aconitase-2

Authors - Ling-Yue Sun
View Article