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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 68253-1-Ig (same clone as 68253-1-PBS)

    WB analysis using 68253-1-Ig (same clone as 68253-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68253-1-Ig (NDUFS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 68253-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 68253-1-Ig (same clone as 68253-1-PBS)

    IHC staining of mouse heart using 68253-1-Ig (same clone as 68253-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68253-1-Ig (NDUFS1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68253-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 68253-1-Ig (same clone as 68253-1-PBS)

    IHC staining of mouse heart using 68253-1-Ig (same clone as 68253-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68253-1-Ig (NDUFS1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68253-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 68253-1-Ig (same clone as 68253-1-PBS)

    IF Staining of HepG2 using 68253-1-Ig (same clone as 68253-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NDUFS1 antibody (68253-1-Ig, Clone: 3H5E8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68253-1-PBS in a different storage buffer formulation.

NDUFS1 Monoclonal antibody, PBS Only

NDUFS1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 68253-1-PBS

产品说明书

CloneNo. 3H5E8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

EC:7.1.1.2, Complex I-75kD, Complex I 75kD, CI-75kD, CI 75Kd

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68253-1-PBS targets NDUFS1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 NDUFS1 fusion protein Ag3135 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase)
别名 EC:7.1.1.2, Complex I-75kD, Complex I 75kD, CI-75kD, CI 75Kd
计算分子量 727 aa, 79 kDa
观测分子量68-81 kDa
GenBank蛋白编号BC030833
基因名称 NDUFS1
Gene ID (NCBI) 4719
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP28331
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The multisubunit NADH:ubiquinone oxidoreductase (75 kDa subunit, mitochondrial)(NDUFS1) is the first enzyme complex in the electron transport chain of mitochondria. It is also named as Complex I-75kD. By use of chaotropic agents, complex I can be fragmented into 3 different fractions: a flavoprotein fraction, an iron-sulfur protein (IP) fraction, and a hydrophobic protein (HP) fraction. NDUFS1 is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. NDUFS1 has some isoforms with the molecular mass of 68-81 kDa.