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NDUFS1 Monoclonal antibody, PBS Only

NDUFS1 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig, Rabbit

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

3H5E8

Cat No : 68253-1-PBS

Print datasheet

Synonyms

CI 75Kd, Complex I 75kD, NDUFS1, PRO1304

验证数据展示

  • WB analysis using 68253-1-Ig (same clone as 68253-1-PBS)

    WB analysis using 68253-1-Ig (same clone as 68253-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68253-1-Ig (NDUFS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 68253-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 68253-1-Ig (same clone as 68253-1-PBS)

    IHC staining of mouse heart using 68253-1-Ig (same clone as 68253-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68253-1-Ig (NDUFS1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68253-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 68253-1-Ig (same clone as 68253-1-PBS)

    IHC staining of mouse heart using 68253-1-Ig (same clone as 68253-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68253-1-Ig (NDUFS1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68253-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 68253-1-Ig (same clone as 68253-1-PBS)

    IF Staining of HepG2 using 68253-1-Ig (same clone as 68253-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NDUFS1 antibody (68253-1-Ig, Clone: 3H5E8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68253-1-PBS in a different storage buffer formulation.

产品信息

68253-1-PBS targets NDUFS1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human, Mouse, Rat, Pig, Rabbit
Immunogen NDUFS1 fusion protein Ag3135 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase)
Synonyms CI 75Kd, Complex I 75kD, NDUFS1, PRO1304
Calculated Molecular Weight 727 aa, 79 kDa
Observed Molecular Weight68-81 kDa
GenBank Accession NumberBC030833
Gene Symbol NDUFS1
Gene ID (NCBI) 4719
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP28331
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

The multisubunit NADH:ubiquinone oxidoreductase (75 kDa subunit, mitochondrial)(NDUFS1) is the first enzyme complex in the electron transport chain of mitochondria. It is also named as Complex I-75kD. By use of chaotropic agents, complex I can be fragmented into 3 different fractions: a flavoprotein fraction, an iron-sulfur protein (IP) fraction, and a hydrophobic protein (HP) fraction. NDUFS1 is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. NDUFS1 has some isoforms with the molecular mass of 68-81 kDa.