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NDUFS2 Polyclonal antibody

NDUFS2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28125-1-AP

Print datasheet

Synonyms

NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial, EC:7.1.1.2, Complex I-49kD, Complex I 49kD, CI-49kD

验证数据展示

  • WB analysis using 28125-1-AP

    WB analysis using 28125-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28125-1-AP (NDUFS2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 28125-1-AP

    IF Staining of HepG2 using 28125-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFS2 antibody (28125-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inJurkat cells, mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28125-1-AP targets NDUFS2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen NDUFS2 fusion protein Ag27078 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase)
Synonyms NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial, EC:7.1.1.2, Complex I-49kD, Complex I 49kD, CI-49kD
Calculated Molecular Weight 53 kDa
Observed Molecular Weight40-50 kDa
GenBank Accession NumberBC000170
Gene Symbol NDUFS2
Gene ID (NCBI) 4720
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75306
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NDUFS2 antibody 28125-1-APDownload protocol
IF protocol for NDUFS2 antibody 28125-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols