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验证数据展示

  • WB analysis using 15224-1-AP (same clone as 15224-1-PBS)

    WB analysis using 15224-1-AP (same clone as 15224-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15224-1-AP (NDUFS5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15224-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 15224-1-AP (same clone as 15224-1-PBS)

    WB analysis of HEK-293 using 15224-1-AP (same clone as 15224-1-PBS)

    WB result of NDUFS5 antibody (15224-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NDUFS5 transfected HEK-293 cells. This data was developed using the same antibody clone with 15224-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 15224-1-AP (same clone as 15224-1-PBS)

    IHC staining of human liver using 15224-1-AP (same clone as 15224-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15224-1-AP (NDUFS5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15224-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

NDUFS5 Polyclonal antibody, PBS Only

NDUFS5 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 15224-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

15224-1-PBS targets NDUFS5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NDUFS5 fusion protein Ag7441 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NADH dehydrogenase (ubiquinone) Fe-S protein 5, 15kDa (NADH-coenzyme Q reductase)
别名
计算分子量 13 kDa
观测分子量 15 kDa
GenBank蛋白编号BC001884
基因名称 NDUFS5
Gene ID (NCBI) 4725
RRIDAB_2149021
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43920
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NDUFS5 gene is a member of the NADH dehydrogenase (ubiquinone) iron-sulfur protein family. The encoded protein is a subunit of the NADH:ubiquinone oxidoreductase (complex I), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane. The NDUFS5 mRNA is expressed ubiquitously in human tissues, with a relative higher expression in human heart, skeletal muscle, liver, kidney and fetal heart (PMID: 10070614). The NDUFS5 (15 kDa) subunit located at mitochondrion and contains two C-X9-C motifs that are predicted to form a helix-coil-helix structure, permitting the formation of intramolecular disulfide bonds (PMID: 21310150).