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NDUFS8 Polyclonal antibody

NDUFS8 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25172-1-AP

Print datasheet

Synonyms

CI 23kD, Complex I 23kD, NDUFS8, TYKY, TYKY subunit

验证数据展示

  • WB analysis of mouse brain using 25172-1-AP

    WB analysis of mouse brain using 25172-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25172-1-AP (NDUFS8 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 25172-1-AP

    IHC staining of human liver cancer using 25172-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25172-1-AP (NDUFS8 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver cancer using 25172-1-AP

    IHC staining of human liver cancer using 25172-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25172-1-AP (NDUFS8 Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inmouse brain tissue, mouse heart tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

25172-1-AP targets NDUFS8 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen NDUFS8 fusion protein Ag18531 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase)
Synonyms CI 23kD, Complex I 23kD, NDUFS8, TYKY, TYKY subunit
Calculated Molecular Weight 210 aa, 24 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC119754
Gene Symbol NDUFS8
Gene ID (NCBI) 4728
RRIDAB_2879939
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00217
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NDUFS8 antibody 25172-1-APDownload protocol
IHC protocol for NDUFS8 antibody 25172-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Biomed Pharmacother

Combination of RNA-seq and proteomics reveals the mechanism of DL0410 treatment in APP/PS1 transgenic mouse model of Alzheimer's disease

Authors - Baoyue Zhang
View Article