验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HEK-293 cells, HeLa cells, HepG2 cells, U2OS cells, mouse heart tissue |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy NDUFV1 Ready-To-Use IHC Kit. NDUFV1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11238-1-AP targets NDUFV1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig |
| 免疫原 | NDUFV1 fusion protein Ag1764 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | NADH dehydrogenase (ubiquinone) flavoprotein 1, 51kDa |
| 别名 | CI 51kD, Complex I 51kD, NDUFV1, UQOR1 |
| 计算分子量 | 51 kDa |
| 观测分子量 | 45 kDa |
| GenBank蛋白编号 | BC015645 |
| 基因名称 | NDUFV1 |
| Gene ID (NCBI) | 4723 |
| RRID | AB_2149040 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P49821 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DUFV1(NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial) is also named as UQOR1,Complex I-51kD and belongs to the complex I 51 kDa subunit family. It is the core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. It has 2 isoforms produced by alternative splicing. Defects in NDUFV1 are a cause of Leigh syndrome (LS) and mitochondrial complex I deficiency (MT-C1D).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NDUFV1 antibody 11238-1-AP | Download protocol |
| IHC protocol for NDUFV1 antibody 11238-1-AP | Download protocol |
| IP protocol for NDUFV1 antibody 11238-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nature In vivo imaging of mitochondrial membrane potential in non-small-cell lung cancer. | ||
Nature Accessory subunits are integral for assembly and function of human mitochondrial complex I. | ||
Mol Cell Sengers Syndrome-Associated Mitochondrial Acylglycerol Kinase Is a Subunit of the Human TIM22 Protein Import Complex. | ||
Brain CLPP deficiency ameliorates neurodegeneration caused by impaired mitochondrial protein synthesis. | ||
Dev Cell Regulation of reverse electron transfer at mitochondrial complex I by unconventional Notch action in cancer stem cells. |