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NDUFV1 Monoclonal antibody

NDUFV1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, pig, rabbit, rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3E9D4

Cat no : 68144-1-Ig

Print datasheet

Synonyms

CI 51kD, Complex I 51kD, NDUFV1, UQOR1

验证数据展示

  • WB analysis using 68144-1-Ig

    WB analysis using 68144-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68144-1-Ig (NDUFV1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 68144-1-Ig

    IHC staining of human lung cancer using 68144-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68144-1-Ig (NDUFV1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NDUFV1 antibody included

经过测试的应用

Positive WB detected inPC-12 cells, A549 cells, 4T1 cells, pig heart tissue, HeLa cells, HEK-293 cells, H9C2 cells, rabbit heart tissue
Positive IHC detected inhuman lung cancer tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy NDUFV1 Ready-To-Use IHC Kit. NDUFV1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68144-1-Ig targets NDUFV1 in WB, IHC, ELISA applications and shows reactivity with Human, pig, rabbit, rat, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, pig, rabbit, rat, mouse
Immunogen NDUFV1 fusion protein Ag32659 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name NADH dehydrogenase (ubiquinone) flavoprotein 1, 51kDa
Synonyms CI 51kD, Complex I 51kD, NDUFV1, UQOR1
Calculated Molecular Weight 51 kDa
Observed Molecular Weight45 kDa
GenBank Accession NumberBC015645
Gene Symbol NDUFV1
Gene ID (NCBI) 4723
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49821
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DUFV1(NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial) is also named as UQOR1,Complex I-51kD and belongs to the complex I 51 kDa subunit family. It is the core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. It has 2 isoforms produced by alternative splicing. Defects in NDUFV1 are a cause of Leigh syndrome (LS) and mitochondrial complex I deficiency (MT-C1D).

实验方案

Product Specific Protocols
WB protocol for NDUFV1 antibody 68144-1-IgDownload protocol
IHC protocol for NDUFV1 antibody 68144-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols