验证数据展示
经过测试的应用
| Positive IHC detected in | human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
19706-1-AP targets NEB-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | nebulin |
| Synonyms | NEB, NEB177D, nebulin, NEM2 |
| Calculated Molecular Weight | 773 kDa |
| GenBank Accession Number | NM_004543 |
| Gene Symbol | NEB |
| Gene ID (NCBI) | 4703 |
| RRID | AB_10637999 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P20929 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NEB, a giant muscle protein, may be involved in maintaining the structural integrity of sarcomeres and the membrane system associated with the myofibrils. NEB binds and stabilizes F-actin. Defects in NEB are the cause of nemaline myopathy type 2 (NEM2). The antibody is specific to NEB.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for NEB-Specific antibody 19706-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Clin Invest KLHL40 deficiency destabilizes thin filament proteins and promotes nemaline myopathy. | ||