验证数据展示
经过测试的应用
Positive IHC detected in | human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
产品信息
19706-1-AP targets NEB-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | IHC, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | nebulin |
别名 | NEB, NEB177D, nebulin, NEM2 |
计算分子量 | 773 kDa |
GenBank蛋白编号 | NM_004543 |
基因名称 | NEB |
Gene ID (NCBI) | 4703 |
RRID | AB_10637999 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P20929 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NEB, a giant muscle protein, may be involved in maintaining the structural integrity of sarcomeres and the membrane system associated with the myofibrils. NEB binds and stabilizes F-actin. Defects in NEB are the cause of nemaline myopathy type 2 (NEM2). The antibody is specific to NEB.
实验方案
Product Specific Protocols | |
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IHC protocol for NEB-Specific antibody 19706-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Clin Invest KLHL40 deficiency destabilizes thin filament proteins and promotes nemaline myopathy. | ||