NEB-Specific Polyclonal antibody
NEB-Specific Polyclonal Antibody for IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive IHC detected in | human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
产品信息
19706-1-AP targets NEB-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IHC, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | nebulin |
Synonyms | NEB, NEB177D, nebulin, NEM2 |
Calculated Molecular Weight | 773 kDa |
GenBank Accession Number | NM_004543 |
Gene Symbol | NEB |
Gene ID (NCBI) | 4703 |
RRID | AB_10637999 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P20929 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NEB, a giant muscle protein, may be involved in maintaining the structural integrity of sarcomeres and the membrane system associated with the myofibrils. NEB binds and stabilizes F-actin. Defects in NEB are the cause of nemaline myopathy type 2 (NEM2). The antibody is specific to NEB.
实验方案
Product Specific Protocols | |
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IHC protocol for NEB-Specific antibody 19706-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Clin Invest KLHL40 deficiency destabilizes thin filament proteins and promotes nemaline myopathy. | ||