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验证数据展示

  • WB analysis using 21497-1-AP

    WB analysis using 21497-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21497-1-AP (NEBL antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 21497-1-AP

    IHC staining of mouse heart using 21497-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21497-1-AP (NEBL antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 21497-1-AP

    IHC staining of mouse heart using 21497-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21497-1-AP (NEBL antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse heart using 21497-1-AP

    IF Staining of mouse heart using 21497-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using NEBL antibody (21497-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NEBL Polyclonal antibody

NEBL Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 21497-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

Nebulette, LNEBL, C10orf113, Actin-binding Z-disk protein, Actin binding Z disk protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse heart tissue, rat heart tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse heart tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21497-1-AP targets NEBL in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NEBL fusion protein Ag15882 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nebulette
别名 Nebulette, LNEBL, C10orf113, Actin-binding Z-disk protein, Actin binding Z disk protein
计算分子量 1014 aa, 116 kDa
观测分子量 116 kDa
GenBank蛋白编号BC110453
基因名称 NEBL
Gene ID (NCBI) 10529
RRIDAB_3085659
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO76041
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nebulette, also known as NEBL, is a cardiac-specific isoform belonging to the nebulin family of proteins. Nebulette has 23 modular repeat structures of 35 amino acid residues each. Nebulette has an acidic region with unknown structure at its N-terminus, and a serine-rich region adjacent to an SH3 domain at its C-terminus (PMID: 2037050, 20951588). Nebulette functionally links sarcomeric actin to the desmin intermediate filaments in the heart muscle sarcomeres (PubMed:27733623). Nebulette is abundantly expressed in cardiac muscle at Z-disc regions, but not in skeletal or smooth muscle.

实验方案

Product Specific Protocols
WB protocol for NEBL antibody 21497-1-APDownload protocol
IHC protocol for NEBL antibody 21497-1-APDownload protocol
IF protocol for NEBL antibody 21497-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols