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NECAB3 Polyclonal antibody

NECAB3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18940-1-AP

Print datasheet

Synonyms

APBA2BP, EFCBP3, NECAB3, Nek2 interacting protein 1, NIP1, STIP3, SYTIP2, X11L binding protein 51, XB51

验证数据展示

  • WB analysis of human brain using 18940-1-AP

    WB analysis of human brain using 18940-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 18940-1-AP (NECAB3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 18940-1-AP

    IHC staining of human pancreas using 18940-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 18940-1-AP (NECAB3 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human pancreas using 18940-1-AP

    IHC staining of human pancreas using 18940-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 18940-1-AP (NECAB3 antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inhuman brain tissue, rat brain tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

18940-1-AP targets NECAB3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen NECAB3 fusion protein Ag13493 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name N-terminal EF-hand calcium binding protein 3
Synonyms APBA2BP, EFCBP3, NECAB3, Nek2 interacting protein 1, NIP1, STIP3, SYTIP2, X11L binding protein 51, XB51
Calculated Molecular Weight362aa,41 kDa; 396aa,44 kDa
Observed Molecular Weight 40-44 kDa
GenBank Accession NumberBC047673
Gene Symbol NECAB3
Gene ID (NCBI) 63941
RRIDAB_10596622
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96P71
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NECAB3, also named as APBA2BP, NIP1, SYTIP2 and XB51, is a potential substrate of Nek2. NECAB3 inhibits the interaction of APBA2 with beta-amyloid precursor protein (APP). NECAB3 has three isoforms with MW 40 kDa, 44 kDa and 22 kDa.

实验方案

Product Specific Protocols
WB protocol for NECAB3 antibody 18940-1-APDownload protocol
IHC protocol for NECAB3 antibody 18940-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

PLoS One

Amyloidosis, Synucleinopathy, and Prion Encephalopathy in a Neuropathic Lysosomal Storage Disease: The CNS-Biomarker Potential of Peripheral Blood.

Authors - Naughton Bartholomew J BJ
View Article