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验证数据展示

  • WB analysis using 83443-6-RR (same clone as 83443-6-PBS)

    WB analysis using 83443-6-RR (same clone as 83443-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83443-6-RR (NEDD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83443-6-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83443-6-RR (same clone as 83443-6-PBS)

    IF Staining of HeLa using 83443-6-RR (same clone as 83443-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NEDD1 antibody (83443-6-RR, Clone: 240404A7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83443-6-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 83443-6-RR (same clone as 83443-6-PBS)

    FC experiment of HEK-293 using 83443-6-RR (same clone as 83443-6-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Nedd1 Recombinant Antibody (83443-6-RR, Clone:240404A7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83443-6-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 83443-6-RR (same clone as 83443-6-PBS)

    FC experiment of A431 using 83443-6-RR (same clone as 83443-6-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Nedd1 Recombinant Antibody (83443-6-RR, Clone:240404A7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83443-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83443-6-RR

    Affinity and Kinetic Characterization of 83443-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83443-6-RR against Human NEDD1 were performed. The affinity constant is below 1 pM.

NEDD1 Recombinant monoclonal antibody, PBS Only

NEDD1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83443-6-PBS

产品说明书

CloneNo. 240404A7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

240404A7, NEDD 1, NEDD-1, Neural precursor cell expressed developmentally down-regulated protein 1, Protein NEDD1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy NEDD1 Ready-To-Use IHC Kit. NEDD1 primary antibody included.

产品信息

83443-6-PBS targets NEDD1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag33603

Product name: Recombinant human NEDD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-332 aa of BC027605

Sequence: MQENLRFASSGDDIKIWDASSMTLVDKFNPHTSPHGISSICWSSNNNFLVTASSSGDKIVVSSCKCKPVPLLELAEGQKQTCVNLNSTSMYLVSGGLNNTVNIWDLKSKRVHRSLKDHKDQVTCVTYNWNDCYIASGSLSGEIILHSVTTNLSSTPFGHGSNQSVRHLKYSLFKKSLLGSVSDNGIVTLWDVNSQSPYHNFDSVHKAPASGICFSPVNELLFVTIGLDKRIILYDTSSKKLVKTLVADTPLTAVDFMPDGATLAIGSSRGKIYQYDLRMLKSPVKTISAHKTSVQCIAFQYSTVLTKSSLNKGCSNKPTTVNKRSVNVNAAS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 neural precursor cell expressed, developmentally down-regulated 1
别名 240404A7, NEDD 1, NEDD-1, Neural precursor cell expressed developmentally down-regulated protein 1, Protein NEDD1
计算分子量 72 kDa
观测分子量71-75 kDa
GenBank蛋白编号BC027605
基因名称 NEDD1
Gene ID (NCBI) 121441
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ8NHV4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NEDD1 (NEDD1 gamma-tubulin ring complex targeting factor), also known as GCP-WD. It is expected to be located in centrosome; nucleoplasm; and plasma membrane. Required for mitosis progression. Promotes the nucleation of microtubules from the spindle. The molecular weight of NEDD1is 72 kDa.

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