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验证数据展示

  • WB analysis using 83112-4-RR (same clone as 83112-4-PBS)

    WB analysis using 83112-4-RR (same clone as 83112-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83112-4-RR (NEDD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83112-4-PBS in a different storage buffer formulation.

  • WB analysis using 83112-4-RR (same clone as 83112-4-PBS)

    WB analysis using 83112-4-RR (same clone as 83112-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83112-4-RR (NEDD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83112-4-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 83112-4-RR (same clone as 83112-4-PBS)

    FC experiment of U2OS using 83112-4-RR (same clone as 83112-4-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug NEDD4 Recombinant antibody (83112-4-RR, Clone:230478D8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83112-4-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 83112-4-RR (same clone as 83112-4-PBS)

    IF Staining of U2OS using 83112-4-RR (same clone as 83112-4-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using NEDD4 antibody (83112-4-RR, Clone: 230478D8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83112-4-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 83112-4-RR (same clone as 83112-4-PBS)

    IF Staining of U2OS using 83112-4-RR (same clone as 83112-4-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using NEDD4 antibody (83112-4-RR, Clone: 230478D8 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83112-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00234-1

    Cytometric bead array standard curve of MP00234-1

    Cytometric bead array standard curve of MP00234-1, NEDD4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83112-2-PBS. Detection antibody: 83112-4-PBS. Standard: Ag16291. Range: 1.25-20 ng/mL.

  • Affinity and Kinetic Characterization of 83112-4-PBS

    Affinity and Kinetic Characterization of 83112-4-PBS

    Biolayer interferometry (BLl) kinetic assay of 83112-4-PBS against Human NEDD4 was performed. The affinity constant is below 1 pM.

  • Affinity and Kinetic Characterization of 83112-4-RR

    Affinity and Kinetic Characterization of 83112-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83112-4-RR against Human NEDD4 were performed. The affinity constant is 73.4 pM.

NEDD4 Recombinant antibody, PBS Only (Detector)

NEDD4 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83112-4-PBS

产品说明书

CloneNo. 230478D8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

230478D8, Cell proliferation-inducing gene 53 protein, E3 ubiquitin-protein ligase NEDD4, EC:2.3.2.26, HECT-type E3 ubiquitin transferase NEDD4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83112-4-PBS targets NEDD4 as part of a matched antibody pair:

MP00234-1: 83112-2-PBS capture and 83112-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NEDD4 fusion protein Ag16291 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 neural precursor cell expressed, developmentally down-regulated 4
别名 230478D8, Cell proliferation-inducing gene 53 protein, E3 ubiquitin-protein ligase NEDD4, EC:2.3.2.26, HECT-type E3 ubiquitin transferase NEDD4
计算分子量 1319 aa, 149 kDa
观测分子量 115 kDa
GenBank蛋白编号BC152452
基因名称 NEDD4
Gene ID (NCBI) 4734
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP46934
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NEDD4 and similar proteins discovered subsequently became a family of HECT ligases, comprising 9 human proteins including NEDD4, NEDD4-2 (NEDD4L), ITCH, SMURF1, SMURF2, WWP1, WWP2, NEDL1 and NEDL2. NEDD4 is a highly evolutionarily conserved protein from yeast to man, and was initially cloned as a highly expressed gene in the early embryonic brain. NEDD4 is frequently overexpressed in many different types of cancer, and decreased levels of NEDD4 can also be associated with cancer. It can be a potential therapeutic target for the treatment of human cancer.(PMID: 25527121). The human NEDD4 gene is located on chromosome 15q21.3 and comprises 30 exons (HGNC:7727) shown to encode a ~120 KDa protein. Otherwise there is a 75 kDa isoform in addition to full length NEDD4 (PMID: 24907641).