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NEDD4L Polyclonal antibody

NEDD4L Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 13690-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (2)

NEDD4.2, KIAA0439, hNEDD4-2, HECT-type E3 ubiquitin transferase NED4L, EC:2.3.2.26

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inC6 cells, HT-1080 cells, PC-3 cells, PC-12 cells
Positive IP detected inC6 cells
Positive IHC detected inhuman prostate cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13690-1-AP targets NEDD4L in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, pig
免疫原 NEDD4L fusion protein Ag4565 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 neural precursor cell expressed, developmentally down-regulated 4-like
别名 NEDD4.2, KIAA0439, hNEDD4-2, HECT-type E3 ubiquitin transferase NED4L, EC:2.3.2.26
计算分子量 854 aa, 96 kDa
观测分子量 112 kDa, 125 kDa
GenBank蛋白编号BC032597
基因名称 NEDD4L
Gene ID (NCBI) 23327
RRIDAB_2149326
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96PU5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NEDD4L(E3 ubiquitin-protein ligase NEDD4-like) is also named as KIAA0439, NEDL3, Nedd4-2. It accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substratesand and is the principal ubiquitin ligase that selectively targets activated SMAD2 and SMAD3 for destruction (PMID:19917253). Nedd4L binds the PY motif of ENaC COOH terminals and catalyzes ubiquitination of the NH2 terminus of the protein for subsequent degradation (PMID:18268134). It has some isoforms produced by alternative splicing. This antibody may have cross reaction with NEDD4 due to the high homology of the antigen.

实验方案

Product Specific Protocols
WB protocol for NEDD4L antibody 13690-1-APDownload protocol
IHC protocol for NEDD4L antibody 13690-1-APDownload protocol
IF protocol for NEDD4L antibody 13690-1-APDownload protocol
IP protocol for NEDD4L antibody 13690-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC,IF

Nat Genet

Mutations in the HECT domain of NEDD4L lead to AKT-mTOR pathway deregulation and cause periventricular nodular heterotopia.

Authors - Loïc Broix
mouseWB

J Exp Med

USP38 critically promotes asthmatic pathogenesis by stabilizing JunB protein.

Authors - Siyuan Chen
ratWB

Cell Death Differ

Aagab acts as a novel regulator of NEDD4-1-mediated Pten nuclear translocation to promote neurological recovery following hypoxic-ischemic brain damage.

Authors - Chunfang Dai
humanWB

Theranostics

JP1 suppresses proliferation and metastasis of melanoma through MEK1/2 mediated NEDD4L-SP1-Integrin αvβ3 signaling.

Authors - Jiahua Cui
rat,humanCoIP,IF

Pharmacol Res

Piezo2 channel in nodose ganglia neurons is essential in controlling hypertension in a pathway regulated directly by Nedd4-2.

Authors - Lifang Huo
mouseWB, IP, IF

Mol Cell Biol

Plk1 Phosphorylation of PTEN Causes a Tumor-Promoting Metabolic State.

Authors - Zhiguo Li
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