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NEDD4L Monoclonal antibody, PBS Only

NEDD4L Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F6H6

Cat no : 67276-1-PBS

Print datasheet

Synonyms

KIAA0439, hNEDD4-2, HECT-type E3 ubiquitin transferase NED4L, EC:2.3.2.26, E3 ubiquitin-protein ligase NEDD4-like

验证数据展示

  • WB analysis of Jurkat using 67276-1-Ig (same clone as 67276-1-PBS)

    WB analysis of Jurkat using 67276-1-Ig (same clone as 67276-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67276-1-Ig (NEDD4L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67276-1-PBS in a different storage buffer formulation.

  • IF Staining of PC-3 using 67276-1-Ig (same clone as 67276-1-PBS)

    IF Staining of PC-3 using 67276-1-Ig (same clone as 67276-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using NEDD4L antibody (67276-1-Ig, Clone: 2F6H6 ) at dilution of 1:3000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67276-1-PBS in a different storage buffer formulation.

产品信息

67276-1-PBS targets NEDD4L in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen NEDD4L fusion protein Ag4565 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name neural precursor cell expressed, developmentally down-regulated 4-like
Synonyms KIAA0439, hNEDD4-2, HECT-type E3 ubiquitin transferase NED4L, EC:2.3.2.26, E3 ubiquitin-protein ligase NEDD4-like
Calculated Molecular Weight 854 aa, 96 kDa
Observed Molecular Weight 112 kDa, 125 kDa
GenBank Accession NumberBC032597
Gene Symbol NEDD4L
Gene ID (NCBI) 23327
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96PU5
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NEDD4L(E3 ubiquitin-protein ligase NEDD4-like) is also named as KIAA0439, NEDL3, Nedd4-2. It accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substratesand and is the principal ubiquitin ligase that selectively targets activated SMAD2 and SMAD3 for destruction (PMID:19917253). Nedd4L binds the PY motif of ENaC COOH terminals and catalyzes ubiquitination of the NH2 terminus of the protein for subsequent degradation (PMID:18268134). It has some isoforms produced by alternative splicing. This antibody may have cross reaction with NEDD4 due to the high homology of the antigen.