• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of Jurkat using 67276-1-Ig (same clone as 67276-1-PBS)

    WB analysis of Jurkat using 67276-1-Ig (same clone as 67276-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67276-1-Ig (NEDD4L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67276-1-PBS in a different storage buffer formulation.

  • IF Staining of PC-3 using 67276-1-Ig (same clone as 67276-1-PBS)

    IF Staining of PC-3 using 67276-1-Ig (same clone as 67276-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using NEDD4L antibody (67276-1-Ig, Clone: 2F6H6 ) at dilution of 1:3000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67276-1-PBS in a different storage buffer formulation.

NEDD4L Monoclonal antibody, PBS Only

NEDD4L Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67276-1-PBS

产品说明书

CloneNo. 2F6H6

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat

应用

WB, IF/ICC, Indirect ELISA

别名

KIAA0439, hNEDD4-2, HECT-type E3 ubiquitin transferase NED4L, EC:2.3.2.26, E3 ubiquitin-protein ligase NEDD4-like

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

67276-1-PBS targets NEDD4L in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 NEDD4L fusion protein Ag4565 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 neural precursor cell expressed, developmentally down-regulated 4-like
别名 KIAA0439, hNEDD4-2, HECT-type E3 ubiquitin transferase NED4L, EC:2.3.2.26, E3 ubiquitin-protein ligase NEDD4-like
计算分子量 854 aa, 96 kDa
观测分子量 112 kDa, 125 kDa
GenBank蛋白编号BC032597
基因名称 NEDD4L
Gene ID (NCBI) 23327
RRIDAB_2882545
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ96PU5
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NEDD4L(E3 ubiquitin-protein ligase NEDD4-like) is also named as KIAA0439, NEDL3, Nedd4-2. It accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substratesand and is the principal ubiquitin ligase that selectively targets activated SMAD2 and SMAD3 for destruction (PMID:19917253). Nedd4L binds the PY motif of ENaC COOH terminals and catalyzes ubiquitination of the NH2 terminus of the protein for subsequent degradation (PMID:18268134). It has some isoforms produced by alternative splicing. This antibody may have cross reaction with NEDD4 due to the high homology of the antigen.