• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83362-2-RR (same clone as 83362-2-PBS)

    WB analysis using 83362-2-RR (same clone as 83362-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83362-2-RR (NEDD9 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83362-2-PBS in a different storage buffer formulation.

  • WB analysis using 83362-2-RR (same clone as 83362-2-PBS)

    WB analysis using 83362-2-RR (same clone as 83362-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83362-2-RR (NEDD9 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83362-2-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 83362-2-RR (same clone as 83362-2-PBS)

    WB analysis of HepG2 using 83362-2-RR (same clone as 83362-2-PBS)

    WB result of NEDD9 antibody (83362-2-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NEDD9 transfected HepG2 cells. This data was developed using the same antibody clone with 83362-2-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 83362-2-RR (same clone as 83362-2-PBS)

    IF Staining of MCF-7 using 83362-2-RR (same clone as 83362-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using NEDD9 antibody (83362-2-RR, Clone: 240310F8 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83362-2-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 83362-2-RR (same clone as 83362-2-PBS)

    IF Staining of A549 using 83362-2-RR (same clone as 83362-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using NEDD9 antibody (83362-2-RR, Clone: 240310F8 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 83362-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00378-2

    Cytometric bead array standard curve of MP00378-2

    Cytometric bead array standard curve of MP00378-2, NEDD9 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83362-2-PBS. Detection antibody: 83362-4-PBS. Standard: Ag34170. Range: 2.5-80 ng/mL.

  • Affinity and Kinetic Characterization of 83362-2-PBS

    Affinity and Kinetic Characterization of 83362-2-PBS

    Biolayer interferometry (BLl) kinetic assay of 83362-2-PBS against Human NEDD9 was performed. The affinity constant is 0.225 nM.

  • Affinity and Kinetic Characterization of 83362-2-RR

    Affinity and Kinetic Characterization of 83362-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83362-2 against Human NEDD9 were performed. The affinity constant is 0.173 nM.

  • Featured Product
  • KD/KO验证

NEDD9 Recombinant antibody, PBS Only (Capture)

NEDD9 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 83362-2-PBS

产品说明书

CloneNo. 240310F8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

HEF1, Enhancer of filamentation 1 p55, CRK-associated substrate-related protein, CAS-L, CASL

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

83362-2-PBS targets NEDD9 as part of a matched antibody pair:

MP00378-2: 83362-2-PBS capture and 83362-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 NEDD9 fusion protein Ag34170 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 neural precursor cell expressed, developmentally down-regulated 9
别名 HEF1, Enhancer of filamentation 1 p55, CRK-associated substrate-related protein, CAS-L, CASL
计算分子量 834 aa, 93 kDa
观测分子量 105 kDa, 115 kDa
GenBank蛋白编号BC040207
基因名称 NEDD9
Gene ID (NCBI) 4739
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ14511
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NEDD9, also known as CASL, HEF1, contains an N-terminal Src homology (SH) 3 domain and an adjacent domain composed of multiple SH2-binding motifs(PMID: 8668148). Cell cycle-regulated processing produces four isoforms: p115, p105, p65, and p55. Isoform p115 arises from p105 phosphorylation and appears later in the cell cycle. Isoform p55 arises from p105 as a result of cleavage at a caspase cleavage-related site and it appears specifically at mitosis. p105 and p115 are predominantly cytoplasmic and associated with focal adhesions, whereas p55associates with the mitotic spindle(PMID: 9584194).