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验证数据展示

  • FC experiment of A549 using 83376-3-RR

    FC experiment of A549 using 83376-3-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Neil1 Recombinant Antibody (83376-3-RR, Clone:240322B7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IHC staining of mouse spleen using 83376-3-RR

    IHC staining of mouse spleen using 83376-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83376-3-RR (NEIL1 antibody) at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 83376-3-RR

    IF Staining of HepG2 using 83376-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NEIL1 antibody (83376-3-RR, Clone: 240322B7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

NEIL1 Recombinant antibody

NEIL1 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83376-3-RR

产品说明书

CloneNo. 240322B7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, FC (Intra), ELISA

别名

NEI L1, Endonuclease VIII-like 1, Endonuclease 8-like 1, EC:3.2.2.-, DNA-(apurinic or apyrimidinic site) lyase Neil1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inA549 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:125-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83376-3-RR targets NEIL1 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 NEIL1 fusion protein Ag2793 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nei endonuclease VIII-like 1 (E. coli)
别名 NEI L1, Endonuclease VIII-like 1, Endonuclease 8-like 1, EC:3.2.2.-, DNA-(apurinic or apyrimidinic site) lyase Neil1
计算分子量 390 aa, 44 kDa
GenBank蛋白编号BC010876
基因名称 NEIL1
Gene ID (NCBI) 79661
RRIDAB_3671028
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96FI4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for NEIL1 antibody 83376-3-RRDownload protocol
IF protocol for NEIL1 antibody 83376-3-RRDownload protocol
FC protocol for NEIL1 antibody 83376-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols