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NEK11 Polyclonal antibody

NEK11 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 12710-1-AP

Print datasheet

Synonyms

NEK11, NimA related protein kinase 11

验证数据展示

  • WB analysis of HeLa using 12710-1-AP

    WB analysis of HeLa using 12710-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12710-1-AP (NEK11 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12710-1-AP targets NEK11 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen NEK11 fusion protein Ag3392 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NIMA (never in mitosis gene a)- related kinase 11
Synonyms NEK11, NimA related protein kinase 11
Calculated Molecular Weight 54 kDa, 74 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC028587
Gene Symbol NEK11
Gene ID (NCBI) 79858
RRIDAB_2150345
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NG66
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NEK11(Serine/threonine-protein kinase) belongs to the NIMA family of kinases, which are involved in DNA replication and genotoxic stress responses.It controls degradation of CDC25A by directly phosphorylating it on residues whose phosphorylation is required for BTRC-mediated polyubiquitination and degradation.It has 4 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for NEK11 antibody 12710-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols