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NEK2 Polyclonal antibody, PBS Only

NEK2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14233-1-PBS

Print datasheet

Synonyms

NimA like protein kinase 1, Never in mitosis A-related kinase 2, NEK2A, HsPK21, HSPK 21

验证数据展示

  • WB analysis using 14233-1-AP (same clone as 14233-1-PBS)

    WB analysis using 14233-1-AP (same clone as 14233-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14233-1-AP (NEK2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14233-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 14233-1-AP (same clone as 14233-1-PBS)

    IF Staining of HeLa using 14233-1-AP (same clone as 14233-1-PBS)

    Immunofluorescent analysis of Hela cells, using NEK2 antibody 14233-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 14233-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 14233-1-AP (same clone as 14233-1-PBS)

    IHC staining of mouse testis using 14233-1-AP (same clone as 14233-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14233-1-AP (NEK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14233-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NEK2 antibody included
  • IHC staining of human skin cancer using 14233-1-AP (same clone as 14233-1-PBS)

    IHC staining of human skin cancer using 14233-1-AP (same clone as 14233-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 14233-1-AP (NEK2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14233-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NEK2 antibody included
Planning an IHC experiment? We recommend our IHCeasy NEK2 Ready-To-Use IHC Kit. NEK2 primary antibody included.

产品信息

14233-1-PBS targets NEK2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen NEK2 fusion protein Ag5474 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NIMA (never in mitosis gene a)-related kinase 2
Synonyms NimA like protein kinase 1, Never in mitosis A-related kinase 2, NEK2A, HsPK21, HSPK 21
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 45-52 kDa
GenBank Accession NumberBC043502
Gene Symbol NEK2
Gene ID (NCBI) 4751
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51955
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C