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验证数据展示

  • WB analysis using 84654-4-RR (same clone as 84654-4-PBS)

    WB analysis using 84654-4-RR (same clone as 84654-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84654-4-RR (NEK4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84654-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 84654-4-RR (same clone as 84654-4-PBS)

    IHC staining of mouse testis using 84654-4-RR (same clone as 84654-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84654-4-RR (NEK4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84654-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NEK4 antibody included
  • IHC staining of mouse testis using 84654-4-RR (same clone as 84654-4-PBS)

    IHC staining of mouse testis using 84654-4-RR (same clone as 84654-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84654-4-RR (NEK4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84654-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NEK4 antibody included
  • IF Staining of mouse testis using 84654-4-RR (same clone as 84654-4-PBS)

    IF Staining of mouse testis using 84654-4-RR (same clone as 84654-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using NEK4 antibody (84654-4-RR, Clone: 242081B9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84654-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84654-4-RR

    Affinity and Kinetic Characterization of 84654-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84654-4-RR against Human NEK4 were performed. The affinity constant is 92.8 pM.

NEK4 Recombinant monoclonal antibody, PBS Only

NEK4 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 84654-4-PBS

产品说明书

CloneNo. 242081B9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, Indirect ELISA

别名

242081B9, NimA related protein kinase 4, NRK2, pp12301, STK2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy NEK4 Ready-To-Use IHC Kit. NEK4 primary antibody included.

产品信息

84654-4-PBS targets NEK4 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag34929

Product name: Recombinant human NEK4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 429-779 aa of BC063044

Sequence: SSDIVTGEKNEPVKPLQPLIKEQKPKDQDQVAGECIIEKQGRIHPDLQPHNSGSEPSLSRQRRQKRREQTEHRGEKRQVRRDLFAFQESPPRFLPSHPIVGKVDVTSTQKEAENQRRVVTGSVSSSRSSEMSSSKDRPLSARERRRLKQSQEEMSSSGPSVRKASLSVAGPGKPQEEDQPLPARRLSSDCSVTQERKQIHCLSEDELSSSTSSTDKSDGDYGEGKGQTNEINALVQLMTQTLKLDSKESCEDVPVANPVSEFKLHRKYRDTLILHGKVAEEAEEIHFKELPSAIMPGSEKIRRLVEVLRTDVIRGLGVQLLEQVYDLLEEEDEFDREVSVSLTVSRCLCYR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 NIMA (never in mitosis gene a)-related kinase 4
别名 242081B9, NimA related protein kinase 4, NRK2, pp12301, STK2
计算分子量 781 aa, 88 kDa
观测分子量 95-100 kDa
GenBank蛋白编号BC063044
基因名称 NEK4
Gene ID (NCBI) 6787
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP51957
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Neks (NIMA-related kinases) are a group of serine-threonine kinases that are related to NIMA, their ortholog from Aspergillus nidulans, which is essential for cells to enter in mitosis. NEK4 was initially identified as STK2, from serine/threonine kinase 2, in a study using a kinase specific cDNA library from human breast cancer tumors or breast cancer cells.

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