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验证数据展示

  • WB analysis using 84654-4-RR (same clone as 84654-4-PBS)

    WB analysis using 84654-4-RR (same clone as 84654-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84654-4-RR (NEK4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84654-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 84654-4-RR (same clone as 84654-4-PBS)

    IHC staining of mouse testis using 84654-4-RR (same clone as 84654-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84654-4-RR (NEK4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84654-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NEK4 antibody included
  • IHC staining of mouse testis using 84654-4-RR (same clone as 84654-4-PBS)

    IHC staining of mouse testis using 84654-4-RR (same clone as 84654-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84654-4-RR (NEK4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84654-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NEK4 antibody included
  • Affinity and Kinetic Characterization of 84654-4-RR

    Affinity and Kinetic Characterization of 84654-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84654-4-RR against Human NEK4 were performed. The affinity constant is 92.8 pM.

NEK4 Recombinant antibody, PBS Only

NEK4 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 84654-4-PBS

产品说明书

CloneNo. 242081B9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

STK2, pp12301, NRK2, NimA related protein kinase 4, 242081B9

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy NEK4 Ready-To-Use IHC Kit. NEK4 primary antibody included.

产品信息

84654-4-PBS targets NEK4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 NEK4 fusion protein Ag34929 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 NIMA (never in mitosis gene a)-related kinase 4
别名 STK2, pp12301, NRK2, NimA related protein kinase 4, 242081B9
计算分子量 781 aa, 88 kDa
观测分子量 95-100 kDa
GenBank蛋白编号BC063044
基因名称 NEK4
Gene ID (NCBI) 6787
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP51957
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Neks (NIMA-related kinases) are a group of serine-threonine kinases that are related to NIMA, their ortholog from Aspergillus nidulans, which is essential for cells to enter in mitosis. NEK4 was initially identified as STK2, from serine/threonine kinase 2, in a study using a kinase specific cDNA library from human breast cancer tumors or breast cancer cells.