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验证数据展示

  • WB analysis using 26254-1-AP

    WB analysis using 26254-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26254-1-AP (NEK5 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human thyroid cancer using 26254-1-AP

    IHC staining of human thyroid cancer using 26254-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 26254-1-AP (NEK5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NEK5 Polyclonal antibody

NEK5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26254-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

NEK5, NimA related protein kinase 5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMDA-MB-231 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26254-1-AP targets NEK5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NEK5 fusion protein Ag24419 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NIMA (never in mitosis gene a)-related kinase 5
别名 NEK5, NimA related protein kinase 5
计算分子量 708 aa, 81 kDa
观测分子量70-80 kDa
GenBank蛋白编号BC063885
基因名称 NEK5
Gene ID (NCBI) 341676
RRIDAB_3085851
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6P3R8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NEK5 antibody 26254-1-APDownload protocol
IHC protocol for NEK5 antibody 26254-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols