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NEK9 Polyclonal antibody

NEK9 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 11192-1-AP

Print datasheet

Synonyms

NimA-related kinase 8, Never in mitosis A-related kinase 9, NERCC, Nek8, KIAA1995

验证数据展示

  • IHC staining of human lymphoma using 11192-1-AP

    IHC staining of human lymphoma using 11192-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11192-1-AP (NEK9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 11192-1-AP

    WB analysis using 11192-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11192-1-AP (NEK9 antibody) at dilution of 1:4500 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 11192-1-AP

    IP experiment of HeLa using 11192-1-AP

    IP result of anti-NEK9 (IP:11192-1-AP, 4ug; Detection:11192-1-AP 1:1000) with HeLa cells lysate 1880 ug.

  • IF Staining of HepG2 using 11192-1-AP

    IF Staining of HepG2 using 11192-1-AP

    Immunofluorescent analysis of HepG2 cells, using NEK9 antibody 11192-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IF Staining of HepG2 using 11192-1-AP

    IF Staining of HepG2 using 11192-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NEK9 antibody (11192-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HeLa using 11192-1-AP

    FC experiment of HeLa using 11192-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human NEK9 (11192-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHeLa cells, human placenta tissue, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:9000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11192-1-AP targets NEK9 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen NEK9 fusion protein Ag1678 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NIMA (never in mitosis gene a)- related kinase 9
Synonyms NimA-related kinase 8, Never in mitosis A-related kinase 9, NERCC, Nek8, KIAA1995
Calculated Molecular Weight 107 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC009336
Gene Symbol NEK9
Gene ID (NCBI) 91754
RRIDAB_2150800
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TD19
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NEK9 is also named as KIAA1995, NEK8, NERCC and belongs to the NEK Ser/Thr protein kinase family. It is originally identified as αβ-casein kinase that associates with a putative substrate Bicd2. It is also independently identified as a Nek6- and Ran GTPase-binding protein under a different name, Nercc1(PMID:14660563). Nek9, together with the highly similar (80% identical) Nek6 and Nek7, form a signaling module that is activated during mitosis and involved in the regulation of the mitotic spindle. The endogenous Nek9 (with predicted molecular mass of 120 kDa) has an apparent molecular mass of 600 kDa, also compatible with a tetramer(PMID:21454704).

实验方案

Product Specific Protocols
WB protocol for NEK9 antibody 11192-1-APDownload protocol
IHC protocol for NEK9 antibody 11192-1-APDownload protocol
IF protocol for NEK9 antibody 11192-1-APDownload protocol
IP protocol for NEK9 antibody 11192-1-APDownload protocol
FC protocol for NEK9 antibody 11192-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

JBMR Plus

Identification of Chloride Intracellular Channel Protein 3 as a Novel Gene Affecting Human Bone Formation.

Authors - Andrea M Brum
View Article
humanWB

Cancers (Basel)

Differential Expression of NEK Kinase Family Members in Esophageal Adenocarcinoma and Barrett's Esophagus

Authors - Lei Chen
View Article