NEK9 Polyclonal antibody
NEK9 Polyclonal Antibody for IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, monkey
Applications
IF/ICC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | COS-7 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
51057-2-AP targets NEK9 in IF/ICC, ELISA applications and shows reactivity with human, monkey samples.
| Tested Applications | IF/ICC, ELISA Application Description |
| Tested Reactivity | human, monkey |
| Immunogen | NEK9 fusion protein Ag1689 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | NIMA (never in mitosis gene a)- related kinase 9 |
| Synonyms | KIAA1995, Nek8, NEK9, NERCC, NERCC1, Nercc1 kinase, NimA related kinase 8, NimA related protein kinase 9 |
| Calculated Molecular Weight | 107 kDa |
| GenBank Accession Number | BC009336 |
| Gene Symbol | NEK9 |
| Gene ID (NCBI) | 91754 |
| RRID | AB_2881241 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TD19 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NEK9 is also named as KIAA1995, NEK8, NERCC and belongs to the NEK Ser/Thr protein kinase family. It is independently identified as a Nek6- and Ran GTPase- binding protein under a different name, Nercc1 (PMID:14660563). Nek9, together with the highly similar Nek6 and Nek7, form a signaling module that is activated during mitosis and involved in the regulation of the mitotic spindle. Confocal microscopy identified that Nek9 was localized to the spindle poles at the metaphase stages and associated with the midbody at anaphase or telophase stage in both meiotic oocytes and the first mitotic embyros (PMID: 23159858). The endogenous Nek9 (with predicted molecular mass of 120 kDa) has an apparent molecular mass of 600 kDa, also compatible with a tetramer (PMID:21454704).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for NEK9 antibody 51057-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |