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验证数据展示

  • IF Staining of COS-7 using 51057-2-AP

    IF Staining of COS-7 using 51057-2-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed COS-7 cells using 51057-2-AP (NEK9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

NEK9 Polyclonal antibody

NEK9 Polyclonal Antibody for IF/ICC, ELISA
Cat No. 51057-2-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, monkey

应用

IF/ICC, ELISA

别名

KIAA1995, Nek8, NEK9, NERCC, NERCC1, Nercc1 kinase, NimA related kinase 8, NimA related protein kinase 9

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inCOS-7 cells
Planning an IHC experiment? We recommend our IHCeasy NEK9 Ready-To-Use IHC Kit. NEK9 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

51057-2-AP targets NEK9 in IF/ICC, ELISA applications and shows reactivity with human, monkey samples.

经测试应用 IF/ICC, ELISA Application Description
经测试反应性 human, monkey
免疫原 NEK9 fusion protein Ag1689 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NIMA (never in mitosis gene a)- related kinase 9
别名 KIAA1995, Nek8, NEK9, NERCC, NERCC1, Nercc1 kinase, NimA related kinase 8, NimA related protein kinase 9
计算分子量 107 kDa
GenBank蛋白编号BC009336
基因名称 NEK9
Gene ID (NCBI) 91754
RRIDAB_2881241
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TD19
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NEK9 is also named as KIAA1995, NEK8, NERCC and belongs to the NEK Ser/Thr protein kinase family. It is independently identified as a Nek6- and Ran GTPase- binding protein under a different name, Nercc1 (PMID:14660563). Nek9, together with the highly similar Nek6 and Nek7, form a signaling module that is activated during mitosis and involved in the regulation of the mitotic spindle. Confocal microscopy identified that Nek9 was localized to the spindle poles at the metaphase stages and associated with the midbody at anaphase or telophase stage in both meiotic oocytes and the first mitotic embyros (PMID: 23159858). The endogenous Nek9 (with predicted molecular mass of 120 kDa) has an apparent molecular mass of 600 kDa, also compatible with a tetramer (PMID:21454704).

实验方案

Product Specific Protocols
IF protocol for NEK9 antibody 51057-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols