验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, human brain tissue, human colon tissue, mouse testis tissue |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
12244-1-AP targets NELF in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | NELF fusion protein Ag2888 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | nasal embryonic LHRH factor |
别名 | NSMF, NMDA receptor synaptonuclear signaling and neuronal migration factor, Nasal embryonic luteinizing hormone-releasing hormone factor, Nasal embryonic LHRH factor |
计算分子量 | 507 aa, 56 kDa |
观测分子量 | 54-60 kDa |
GenBank蛋白编号 | BC016862 |
基因名称 | NELF |
Gene ID (NCBI) | 26012 |
RRID | AB_2150819 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q6X4W1 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NSMF, also named as NELF (Nasal embryonic LHRH factor), couples NMDA-sensitive glutamate receptor signaling to the nucleus and triggers long-lasting changes in the cytoarchitecture of dendrites and spine synapse processes. NSMF has some isoforms with MW 44 kDa and 55-60 kDa. This antibody recognizes all the isoforms.
实验方案
Product Specific Protocols | |
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WB protocol for NELF antibody 12244-1-AP | Download protocol |
IHC protocol for NELF antibody 12244-1-AP | Download protocol |
IF protocol for NELF antibody 12244-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |