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Neudesin/NENF Polyclonal antibody

Neudesin/NENF Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 15760-1-AP

Print datasheet

Synonyms

Neudesin / NENF, Neudesin, NENF, Protein GIG47, Neuron-derived neurotrophic factor

验证数据展示

  • WB analysis using 15760-1-AP

    WB analysis using 15760-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15760-1-AP (Neudesin/NENF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHCT 116 cells, HEK-293 cells, HeLa cells, MCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy NENF Ready-To-Use IHC Kit. NENF primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15760-1-AP targets Neudesin/NENF in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Neudesin/NENF fusion protein Ag8387 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name neuron derived neurotrophic factor
Synonyms Neudesin / NENF, Neudesin, NENF, Protein GIG47, Neuron-derived neurotrophic factor
Calculated Molecular Weight 172 aa, 19 kDa
Observed Molecular Weight19 kDa
GenBank Accession NumberBC008823
Gene Symbol NENF
Gene ID (NCBI) 29937
RRIDAB_2150989
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UMX5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neudesin neurotrophic factor (NENF, also known as CIR2, SPUF, and SCIRP10) acts as a neurotrophic factor in postnatal mature neurons enhancing neuronal survival, which is localized to mitochondria and endoplasmic reticulum by PINK1 and PARK7 (PMID: 31536960). NENF in the adult brain is expected to play roles in the maintenance and protection of neurons in an autocrine/paracrine manner (PMID: 15605373). It greatly increases cAMP levels in neural precursor cells and might activate a Gs-protein-coupled receptor that could activate the MAPK, PKA, and PI-3K signal pathways (PMID: 16547973).

实验方案

Product Specific Protocols
WB protocol for Neudesin/NENF antibody 15760-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols