Neudesin/NENF Monoclonal antibody
Neudesin/NENF Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, pig
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
4G9E12
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, human brain tissue, HepG2 cells, LNCaP cells, A549 cells, HCT 116 cells, Jurkat cells, pig brain tissue |
| Positive IHC detected in | human cerebellum tissue, human colon tissue, human colon cancer tissue, human heart tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy NENF Ready-To-Use IHC Kit. NENF primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
60131-1-Ig targets Neudesin/NENF in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, pig |
| Cited Reactivity | human |
| Immunogen | Neudesin/NENF fusion protein Ag8387 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | neuron derived neurotrophic factor |
| Synonyms | Neudesin, NENF, Protein GIG47, Neuron-derived neurotrophic factor, Cell immortalization-related protein 2 |
| Calculated Molecular Weight | 172 aa, 19 kDa |
| Observed Molecular Weight | 19 kDa, 16 kDa |
| GenBank Accession Number | BC008823 |
| Gene Symbol | NENF |
| Gene ID (NCBI) | 29937 |
| RRID | AB_2150987 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9UMX5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Neudesin neurotrophic factor (NENF, also known as CIR2, SPUF, and SCIRP10) acts as a neurotrophic factor in postnatal mature neurons enhancing neuronal survival, which is localized to mitochondria and endoplasmic reticulum by PINK1 and PARK7 (PMID: 31536960). NENF in the adult brain is expected to play roles in the maintenance and protection of neurons in an autocrine/paracrine manner (PMID: 15605373). It greatly increases cAMP levels in neural precursor cells and might activate a Gs-protein-coupled receptor that could activate the MAPK, PKA, and PI-3K signal pathways (PMID: 16547973).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Neudesin/NENF antibody 60131-1-Ig | Download protocol |
| IHC protocol for Neudesin/NENF antibody 60131-1-Ig | Download protocol |
| IF protocol for Neudesin/NENF antibody 60131-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
