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NET1 Polyclonal antibody

NET1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12740-1-AP

Print datasheet

Synonyms

Proto-oncogene p65 Net1, Proto oncogene p65 Net1, Neuroepithelial cell-transforming gene 1 protein, NET1A, ARHGEF8

验证数据展示

  • WB analysis of Y79 using 12740-1-AP

    WB analysis of Y79 using 12740-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 12740-1-AP (NET1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 12740-1-AP

    IHC staining of human stomach using 12740-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12740-1-AP (NET1 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human stomach using 12740-1-AP

    IHC staining of human stomach using 12740-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12740-1-AP (NET1 antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of A431 using 12740-1-AP

    IF Staining of A431 using 12740-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using NET1 antibody (12740-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inY79 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

12740-1-AP targets NET1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen NET1 fusion protein Ag3636 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name neuroepithelial cell transforming 1
Synonyms Proto-oncogene p65 Net1, Proto oncogene p65 Net1, Neuroepithelial cell-transforming gene 1 protein, NET1A, ARHGEF8
Calculated Molecular Weight 596 aa, 68 kDa
Observed Molecular Weight68 kDa
GenBank Accession NumberBC010285
Gene Symbol NET1
Gene ID (NCBI) 10276
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z628
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NET1 antibody 12740-1-APDownload protocol
IHC protocol for NET1 antibody 12740-1-APDownload protocol
IF protocol for NET1 antibody 12740-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Hum Pathol

Short interfering RNA targeting Net1 reduces the angiogenesis and tumor growth of in vivo cervical squamous cell carcinoma through VEGF down-regulation.

Authors - Yuting Zhang
View Article