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NEU2 Recombinant antibody, PBS Only

NEU2 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240685B3

Cat no : 83934-1-PBS

Print datasheet

Synonyms

SIAL2, N-acetyl-alpha-neuraminidase 2, N acetyl alpha neuraminidase 2, EC:3.2.1.18, Cytosolic sialidase

验证数据展示

  • WB analysis of mouse skeletal muscle using 83934-1-RR (same clone as 83934-1-PBS)

    WB analysis of mouse skeletal muscle using 83934-1-RR (same clone as 83934-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 83934-1-RR (NEU2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83934-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 83934-1-RR (same clone as 83934-1-PBS)

    IHC staining of mouse skeletal muscle using 83934-1-RR (same clone as 83934-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 83934-1-RR (NEU2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83934-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 83934-1-RR (same clone as 83934-1-PBS)

    IHC staining of mouse skeletal muscle using 83934-1-RR (same clone as 83934-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 83934-1-RR (NEU2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83934-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83934-1-RR

    Affinity and Kinetic Characterization of 83934-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83934-1-RR against Human NEU2 were performed. The affinity constant is 2.76 nM.

产品信息

The immunogen of 83934-1-PBS is NEU2 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen NEU2 fusion protein Ag17386 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name sialidase 2 (cytosolic sialidase)
Synonyms SIAL2, N-acetyl-alpha-neuraminidase 2, N acetyl alpha neuraminidase 2, EC:3.2.1.18, Cytosolic sialidase
Calculated Molecular Weight 380 aa, 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC107053
Gene Symbol NEU2
Gene ID (NCBI) 4759
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9Y3R4
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NEU2, also named as Sialidase 2, neuraminidase 2, catalyzes the hydrolytic cleavage of the terminal sialic acid (N-acetylneuraminic acid, Neu5Ac) of a glycan moiety in the catabolism of glycolipids, glycoproteins and oligosaccharides. NEU2 is localized in the cytosol and plasma membrane and takes part in myoblast and neuronal differentiation. It is almost undetectable under normal physiological conditions, and was found to be involved into apoptotic events in pancreatic ductal adenocarcinoma, one of the most dangerous cancers due to poor diagnosis and prognosis. (PMID: 30439363)