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验证数据展示

  • WB analysis of HEK-293 using 66642-1-Ig

    WB analysis of HEK-293 using 66642-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66642-1-Ig (NEUROG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of rat brain using 66642-1-Ig

    IF Staining of rat brain using 66642-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NEUROG1 antibody (66642-1-Ig, Clone: 1E1H6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NEUROG1 Monoclonal antibody

NEUROG1 Monoclonal Antibody for WB, IF-P, ELISA
Cat No. 66642-1-Ig
CloneNo. 1E1H6

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF-P, ELISA

Synonyms

NEUROD3, Math4C, Class A basic helix-loop-helix protein 6, bHLHa6, AKA

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, Y79 cells, Neuro-2a cells, HeLa cells, mouse brain tissue
Positive IF-P detected inrat brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66642-1-Ig targets NEUROG1 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF-P, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen NEUROG1 fusion protein Ag26438 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name neurogenin 1
Synonyms NEUROD3, Math4C, Class A basic helix-loop-helix protein 6, bHLHa6, AKA
Calculated Molecular Weight 237 aa, 26 kDa
Observed Molecular Weight 20-25 kDa
GenBank Accession NumberBC008687
Gene Symbol NEUROG1
Gene ID (NCBI) 4762
RRIDAB_2882001
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92886
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NEUROG1, also named BHLHA6, NEUROD3, NGN, and NGN1, is a 237 amino acid protein, which is expressed in embryonic nervous system. NEUROG1 acts as a transcriptional regulator that is involved in the initiation of neuronal differentiation. It activates transcription by binding to the E box (5'-CANNTG-3'). NEUROG1 can associate with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis.

实验方案

Product Specific Protocols
WB protocol for NEUROG1 antibody 66642-1-IgDownload protocol
IF protocol for NEUROG1 antibody 66642-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols