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NF2 Polyclonal antibody

NF2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10659-1-AP

Print datasheet

Synonyms

Neurofibromin-2, Neurofibromin 2, Moesin-ezrin-radixin-like protein, Merlin

验证数据展示

  • WB analysis using 10659-1-AP

    WB analysis using 10659-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10659-1-AP (NF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse kidney using 10659-1-AP

    WB analysis of mouse kidney using 10659-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10659-1-AP (NF2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 10659-1-AP

    IHC staining of human stomach using 10659-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10659-1-AP (NF2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NF2 antibody included

经过测试的应用

Positive WB detected inJurkat cells, human kidney tissue, mouse kidney tissue, NIH/3T3 cells, rat kidney tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy NF2 Ready-To-Use IHC Kit. NF2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

10659-1-AP targets NF2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen NF2 fusion protein Ag1013 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name neurofibromin 2 (merlin)
Synonyms Neurofibromin-2, Neurofibromin 2, Moesin-ezrin-radixin-like protein, Merlin
Calculated Molecular Weight 70 kDa
Observed Molecular Weight 64-66 kDa
GenBank Accession NumberBC007279
Gene Symbol NF2
Gene ID (NCBI) 4771
RRIDAB_2149815
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35240
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neurofibromatosis 2 (NF2) tumor suppressor protein-merlin is involved in Hippo/SWH signaling pathway that associated with tumor suppression by restricting proliferation and promoting apoptosis. It has been reported that merlin can change to phosphorylation form which inactivates merlin tumor suppressive abilities at serine 518 induced by Rac effector p21-activated kinase 2. The mutation of the NF2 tumor suppressor gene may lead to nervous system tumors, mesotheliomas and metastatic tumors.

实验方案

Product Specific Protocols
WB protocol for NF2 antibody 10659-1-APDownload protocol
IHC protocol for NF2 antibody 10659-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Exp Clin Cancer Res

The histone lysine acetyltransferase KAT2B inhibits cholangiocarcinoma growth: evidence for interaction with SP1 to regulate NF2-YAP signaling

Authors - Wenbo Ma
View Article