验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, human kidney tissue, mouse kidney tissue, NIH/3T3 cells, rat kidney tissue |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy NF2 Ready-To-Use IHC Kit. NF2 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
10659-1-AP targets Neurofibromin 2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Immunogen | Neurofibromin 2 fusion protein Ag1013 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | neurofibromin 2 (merlin) |
| Synonyms | NF2, Neurofibromin-2, Moesin-ezrin-radixin-like protein, Merlin |
| Calculated Molecular Weight | 70 kDa |
| Observed Molecular Weight | 64-66 kDa |
| GenBank Accession Number | BC007279 |
| Gene Symbol | NF2 |
| Gene ID (NCBI) | 4771 |
| RRID | AB_2149815 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P35240 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Neurofibromatosis 2 (NF2) tumor suppressor protein-merlin is involved in Hippo/SWH signaling pathway that associated with tumor suppression by restricting proliferation and promoting apoptosis. It has been reported that merlin can change to phosphorylation form which inactivates merlin tumor suppressive abilities at serine 518 induced by Rac effector p21-activated kinase 2. The mutation of the NF2 tumor suppressor gene may lead to nervous system tumors, mesotheliomas and metastatic tumors.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Neurofibromin 2 antibody 10659-1-AP | Download protocol |
| IHC protocol for Neurofibromin 2 antibody 10659-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
