- Featured Product
- KD/KO Validated
Neurofibromin 2 Polyclonal antibody
Neurofibromin 2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, canine
Applications
WB, IHC, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HepG2 cells, MCF-7 cells, HEK-293 cells, Jurkat cells, SH-SY5Y cells, NIH/3T3 cells, MDCK cells, mouse brain tissue, rat brain tissue |
Positive IP detected in | HeLa cells |
Positive IHC detected in | mouse brain tissue, human meningioma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:750-1:3000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
21686-1-AP targets Neurofibromin 2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse, rat, canine |
Cited Reactivity | human, mouse, rat |
Immunogen | Neurofibromin 2 fusion protein Ag16265 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | neurofibromin 2 (merlin) |
Synonyms | NF2, Neurofibromin-2, Moesin-ezrin-radixin-like protein, Merlin |
Calculated Molecular Weight | 595 aa, 70 kDa |
Observed Molecular Weight | 66-70 kDa |
GenBank Accession Number | BC020257 |
Gene Symbol | NF2 |
Gene ID (NCBI) | 4771 |
RRID | AB_10792410 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P35240 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Neurofibromin 2 antibody 21686-1-AP | Download protocol |
IHC protocol for Neurofibromin 2 antibody 21686-1-AP | Download protocol |
IF protocol for Neurofibromin 2 antibody 21686-1-AP | Download protocol |
IP protocol for Neurofibromin 2 antibody 21686-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Thyroid MicroRNA-146b-3p promotes cell metastasis by directly targeting NF2 in human papillary thyroid cancer. | ||
Onco Targets Ther PWP1 Promotes the Malignant Phenotypes of Lung Cancer Cells by Interacting with DVL2 and Merlin.
| ||
Cancer Manag Res MiR-25-3p Serves as an Oncogenic MicroRNA by Downregulating the Expression of Merlin in Osteosarcoma. | ||
Biochem Biophys Res Commun A hairpin within YAP mRNA 3'UTR functions in regulation at post-transcription level. | ||
J Cell Physiol Enhancement of tenogenic differentiation of rat tendon-derived stem cells by biglycan. |