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Neurofibromin 2 Polyclonal antibody

Neurofibromin 2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 21686-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, canine

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

NF2, Neurofibromin-2, Moesin-ezrin-radixin-like protein, Merlin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, HEK-293 cells, Jurkat cells, SH-SY5Y cells, NIH/3T3 cells, MDCK cells, mouse brain tissue, rat brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inmouse brain tissue, human meningioma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NF2 Ready-To-Use IHC Kit. NF2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:750-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21686-1-AP targets Neurofibromin 2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat, canine
文献引用反应性human, mouse, rat
免疫原 Neurofibromin 2 fusion protein Ag16265 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 neurofibromin 2 (merlin)
别名 NF2, Neurofibromin-2, Moesin-ezrin-radixin-like protein, Merlin
计算分子量 595 aa, 70 kDa
观测分子量 66-70 kDa
GenBank蛋白编号BC020257
基因名称 NF2
Gene ID (NCBI) 4771
RRIDAB_10792410
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP35240
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neurofibromatosis 2 (NF2) tumor suppressor protein-merlin is involved in Hippo/SWH signaling pathway that associated with tumor suppression by restricting proliferation and promoting apoptosis. It has been reported that merlin can change to phosphorylation form which inactivates merlin tumor suppressive abilities at serine 518 induced by Rac effector p21-activated kinase 2. The mutation of the NF2 tumor suppressor gene may lead to nervous system tumors, mesotheliomas and metastatic tumors.

实验方案

Product Specific Protocols
WB protocol for Neurofibromin 2 antibody 21686-1-APDownload protocol
IHC protocol for Neurofibromin 2 antibody 21686-1-APDownload protocol
IF protocol for Neurofibromin 2 antibody 21686-1-APDownload protocol
IP protocol for Neurofibromin 2 antibody 21686-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB

Thyroid

MicroRNA-146b-3p promotes cell metastasis by directly targeting NF2 in human papillary thyroid cancer.

Authors - Chunxiao Yu
humanWB

J Cell Mol Med

PMID: 32281270

Authors - Jie Liu
humanIF

Onco Targets Ther

PWP1 Promotes the Malignant Phenotypes of Lung Cancer Cells by Interacting with DVL2 and Merlin.

Authors - Lai Wei
  • KD Validated
humanWB

Cancer Manag Res

MiR-25-3p Serves as an Oncogenic MicroRNA by Downregulating the Expression of Merlin in Osteosarcoma.

Authors - Hua-Chun Rao
humanWB

Biochem Biophys Res Commun

A hairpin within YAP mRNA 3'UTR functions in regulation at post-transcription level.

Authors - Yuen Gao
ratWB

J Cell Physiol

Enhancement of tenogenic differentiation of rat tendon-derived stem cells by biglycan.

Authors - Yan-Jing Zhang
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