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NF45 Polyclonal antibody

NF45 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24572-1-AP

Print datasheet

Synonyms

ILF2, PRO3063, Nuclear factor of activated T-cells 45 kDa, Interleukin enhancer-binding factor 2

验证数据展示

  • WB analysis of K-562 using 24572-1-AP

    WB analysis of K-562 using 24572-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 24572-1-AP (NF45 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 24572-1-AP

    IF Staining of HepG2 using 24572-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 24572-1-AP (NF45 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human colon using 24572-1-AP

    IHC staining of human colon using 24572-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 24572-1-AP (NF45 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inK-562 cells, HepG2 cells
Positive IHC detected inhuman colon tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24572-1-AP targets NF45 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen NF45 fusion protein Ag21480 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name interleukin enhancer binding factor 2, 45kDa
Synonyms ILF2, PRO3063, Nuclear factor of activated T-cells 45 kDa, Interleukin enhancer-binding factor 2
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC000382
Gene Symbol ILF2
Gene ID (NCBI) 3608
RRIDAB_2879615
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12905
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Interleukin-2 enhancer binding factor 2 (ILF2), also named as nuclear factor 45 (NF45), plays important roles in regulating interleukin-2 expression in mammals. It forms a heterodimeric complex with ILF3, which may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA [PMID:11739746,17129403].

实验方案

Product Specific Protocols
WB protocol for NF45 antibody 24572-1-APDownload protocol
IHC protocol for NF45 antibody 24572-1-APDownload protocol
IF protocol for NF45 antibody 24572-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols