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验证数据展示

  • WB analysis of rat brain using 26351-1-AP

    WB analysis of rat brain using 26351-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 26351-1-AP (Neurofascin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 26351-1-AP

    IHC staining of human brain using 26351-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26351-1-AP (Neurofascin antibody at dilution of 1:200 (under 10x lens).

  • IHC staining of human brain using 26351-1-AP

    IHC staining of human brain using 26351-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26351-1-AP (Neurofascin antibody at dilution of 1:200 (under 40x lens).

Neurofascin Polyclonal antibody

Neurofascin Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26351-1-AP

产品说明书

引用文献(4)

宿主/亚型

Rabbit / IgG

种属反应性

human, rat, mouse

应用

WB, IHC, ELISA

别名

KIAA0756, Neurofascin, neurofascin homolog (chicken), NFASC, NRCAML

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 4 publications below

产品信息

26351-1-AP targets Neurofascin in WB, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, rat, mouse
文献引用反应性human, mouse, rat
免疫原 Neurofascin fusion protein Ag24165 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 neurofascin homolog (chicken)
别名 KIAA0756, Neurofascin, neurofascin homolog (chicken), NFASC, NRCAML
计算分子量 1347 aa, 150 kDa
观测分子量 186 kDa
GenBank蛋白编号BC137013
基因名称 Neurofascin
Gene ID (NCBI) 23114
RRIDAB_2880486
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO94856
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Neurofascin antibody 26351-1-APDownload protocol
IHC protocol for Neurofascin antibody 26351-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Pharmacol Res

The role of circTmeff-1 in incubation of context-induced morphine craving.

Authors - Hailei Yu
View Article
humanWB

Aging (Albany NY)

Tumor-suppressive miR-3650 inhibits tumor metastasis by directly targeting NFASC in hepatocellular carcinoma.

Authors - Jian Wu
View Article
ratWB

J Cancer Prev

Gene Expression Changes by Diallyl Trisulfide Administration in Chemically-induced Mammary Tumors in Rats.

Authors - Eun-Ryeong Hahm
View Article
humanWB

Cell Rep Med

Clinical-proteomic classification and precision treatment strategy of chordoma

Authors - Huabin Yin
View Article