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Neurofascin Polyclonal antibody

Neurofascin Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26351-1-AP

Print datasheet

Synonyms

KIAA0756, Neurofascin, neurofascin homolog (chicken), NFASC, NRCAML

验证数据展示

  • WB analysis of rat brain using 26351-1-AP

    WB analysis of rat brain using 26351-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 26351-1-AP (Neurofascin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 26351-1-AP

    IHC staining of human brain using 26351-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26351-1-AP (Neurofascin antibody at dilution of 1:200 (under 10x lens).

  • IHC staining of human brain using 26351-1-AP

    IHC staining of human brain using 26351-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26351-1-AP (Neurofascin antibody at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inrat brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 4 publications below

产品信息

The immunogen of 26351-1-AP is Neurofascin Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, rat, mouse
Cited Reactivityhuman, mouse, rat
Immunogen Neurofascin fusion protein Ag24165 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name neurofascin homolog (chicken)
Synonyms KIAA0756, Neurofascin, neurofascin homolog (chicken), NFASC, NRCAML
Calculated Molecular Weight 1347 aa, 150 kDa
Observed Molecular Weight 186 kDa
GenBank Accession NumberBC137013
Gene Symbol Neurofascin
Gene ID (NCBI) 23114
RRIDAB_2880486
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO94856
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Neurofascin antibody 26351-1-APDownload protocol
IHC protocol for Neurofascin antibody 26351-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Pharmacol Res

The role of circTmeff-1 in incubation of context-induced morphine craving.

Authors - Hailei Yu
View Article
humanWB

Aging (Albany NY)

Tumor-suppressive miR-3650 inhibits tumor metastasis by directly targeting NFASC in hepatocellular carcinoma.

Authors - Jian Wu
View Article
ratWB

J Cancer Prev

Gene Expression Changes by Diallyl Trisulfide Administration in Chemically-induced Mammary Tumors in Rats.

Authors - Eun-Ryeong Hahm
View Article
humanWB

Cell Rep Med

Clinical-proteomic classification and precision treatment strategy of chordoma

Authors - Huabin Yin
View Article