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NFAT5 Polyclonal antibody

NFAT5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21713-1-AP

Print datasheet

Synonyms

Nuclear factor of activated T-cells 5, NFATZ, NFATL1, NF-AT5, NF AT5

验证数据展示

  • WB analysis using 21713-1-AP

    WB analysis using 21713-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21713-1-AP (NFAT5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 21713-1-AP

    IHC staining of mouse skeletal muscle using 21713-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NFAT5 antibody included
  • IHC staining of mouse skeletal muscle using 21713-1-AP

    IHC staining of mouse skeletal muscle using 21713-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NFAT5 antibody included
  • IF Staining of HepG2 using 21713-1-AP

    IF Staining of HepG2 using 21713-1-AP

    Immunofluorescent analysis of HepG2 cells using 21713-1-AP (NFAT5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, U2OS cells, COS-7 cells
Positive IHC detected inmouse skeletal muscle tissue, human urothelial carcinoma tissue, human thyroid cancer tissue, mouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy NFAT5 Ready-To-Use IHC Kit. NFAT5 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21713-1-AP targets NFAT5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat, monkey
Immunogen NFAT5 fusion protein Ag16341 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nuclear factor of activated T-cells 5, tonicity-responsive
Synonyms Nuclear factor of activated T-cells 5, NFATZ, NFATL1, NF-AT5, NF AT5
Calculated Molecular Weight 1531 aa, 166 kDa
Observed Molecular Weight200 kDa
GenBank Accession NumberBC146765
Gene Symbol NFAT5
Gene ID (NCBI) 10725
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO94916
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NFAT5 (Nuclear factor of activated T-cells 5) is the member of the Rel family of transcription factors, including NF-κB and NFAT1-4, which plays central roles in inducible gene expression during the immune response (PMID: 21629436). NFAT5 also acts as a novel regulator of mammalian anti-pathogen responses, regulating TLR-induced gene expression in primary macrophages induced by Toll-like receptors (PMID: 22312110). NFAT5 is involved in the transcriptional regulation of osmoprotective and inflammatory genes (PMID: 10377394).

实验方案

Product Specific Protocols
WB protocol for NFAT5 antibody 21713-1-APDownload protocol
IHC protocol for NFAT5 antibody 21713-1-APDownload protocol
IF protocol for NFAT5 antibody 21713-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols