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验证数据展示

  • WB analysis using 30114-1-AP

    WB analysis using 30114-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30114-1-AP (NFATC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of U-251 using 30114-1-AP

    IF Staining of U-251 using 30114-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using NFATC1 antibody (30114-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

NFATC1 Polyclonal antibody

NFATC1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30114-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

NF ATC, NF ATc1, NFAT2, NFATc, NFATC1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells
Positive IF/ICC detected inU-251 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30114-1-AP targets NFATC1 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 NFATC1 fusion protein Ag32365 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1
别名 NF ATC, NF ATc1, NFAT2, NFATc, NFATC1
计算分子量716aa,78 kDa; 943aa,101 kDa
观测分子量82-140 kDa
GenBank蛋白编号BC104753
基因名称 NFATC1
Gene ID (NCBI) 4772
RRIDAB_3086233
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95644
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nuclear factor of activated T-cells cytoplasmic (NFATC) is a family of transcription factors originally identified in T-cells. The family has five members (NFATC1 through NFATC5), which play roles both within and outside the immune system. NFATC1 is widely expressed and resides in endocardial cushion endothelial cells, skeletal muscle fibers, T lymphocytes, osteoblasts, osteoclasts, etc. The autoregulation of NFATC1 is a crucial step for cell fate determination. It is a master regulator of RANKL-induced osteoclast differentiation and plays a pivotal role in osteoclast fusion and osteoclast activation via up-regulation of various genes responsible for osteoclast adhesion, migration, acidification, degradation of inorganic and organic bone matrix (PMID: 20035895, PMID: 19754901). NFAT proteins is alternatively modified to generate splice variants. NFATC1 can be detected isoforms of 82-140 kDa (PMID: 18097033).

实验方案

Product Specific Protocols
WB protocol for NFATC1 antibody 30114-1-APDownload protocol
IF protocol for NFATC1 antibody 30114-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols