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  • Featured Product
  • KD/KO Validated

NFATC1 Monoclonal antibody, PBS Only

NFATC1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E1B10

Cat no : 66963-1-PBS

Print datasheet

Synonyms

Nuclear factor of activated T cells, cytoplasmic 1, NF-ATc1, NF-ATc, NFATc, NFAT2

验证数据展示

  • WB analysis using 66963-1-Ig (same clone as 66963-1-PBS)

    WB analysis using 66963-1-Ig (same clone as 66963-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66963-1-Ig (NFATC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66963-1-PBS in a different storage buffer formulation.

  • IHC staining of human urothelial carcinoma using 66963-1-Ig (same clone as 66963-1-PBS)

    IHC staining of human urothelial carcinoma using 66963-1-Ig (same clone as 66963-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 66963-1-Ig (NFATC1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66963-1-PBS in a different storage buffer formulation.

  • IHC staining of human urothelial carcinoma using 66963-1-Ig (same clone as 66963-1-PBS)

    IHC staining of human urothelial carcinoma using 66963-1-Ig (same clone as 66963-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 66963-1-Ig (NFATC1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66963-1-PBS in a different storage buffer formulation.

  • WB analysis using 66963-1-Ig (same clone as 66963-1-PBS)

    WB analysis using 66963-1-Ig (same clone as 66963-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66963-1-Ig (NFATC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66963-1-PBS in a different storage buffer formulation.

产品信息

66963-1-PBS targets NFATC1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen NFATC1 fusion protein Ag13014 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1
Synonyms Nuclear factor of activated T cells, cytoplasmic 1, NF-ATc1, NF-ATc, NFATc, NFAT2
Calculated Molecular Weight716aa,78 kDa; 943aa,101 kDa
Observed Molecular Weight 101 kDa
GenBank Accession NumberBC104753
Gene Symbol NFATC1
Gene ID (NCBI) 4772
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95644
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NFATc1(nuclear factor-activated T cells c1) is one of the transcription factors family consisting 5 members which were considered as a regulator of T-cell activation. In immune cell, NFATc1 is located in the cytoplasm in a hyperphosphorylated state and translocates into the nucleus upon cell stimulation via dephosphorylation(PMID:28327458). One paper observed that ectopic expression and nuclear accumulation of NFATc1 in more than 60% of human pancreatic cancers (PMID:25623042). Several alternative splicing transcript variants encoding different isoforms have been identified for this gene. And different isoforms of this protein may regulate different cytokine gene expression. NFATc1 is a main transcriptional activator for cytokine genes in activated lymphoid cells. Also some studies demonstrated that NFATc1 plays the role of a master transcription regulator of osteoclast differentiation (PMID:25489571).