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NFATC3 Polyclonal antibody

NFATC3 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 18222-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (2)

应用

WB, IP, IHC, ELISA and More (2)

NF AT4, NF ATc3, NFAT4, NFATC3, NFATX

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inJurkat cells, K-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman ovary tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy NFATC3 Ready-To-Use IHC Kit. NFATC3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18222-1-AP targets NFATC3 in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IHC, IF, IP, chIP
经测试反应性 human
文献引用反应性human, mouse, rat
免疫原 NFATC3 fusion protein Ag12985 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3
别名 NF AT4, NF ATc3, NFAT4, NFATC3, NFATX
计算分子量 116 kDa
观测分子量 130-170 kDa
GenBank蛋白编号BC001050
基因名称 NFATC3
Gene ID (NCBI) 4775
RRIDAB_2152773
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ12968
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NFAT (nuclear factors of activated T cells) proteins are a family of transcription factors originally identified as mediators of activation of cytokine genes in response to antigenic stimulation of T cells. NFAT proteins also play varied roles in cells outside of the immune system [PMID:11877454]. NFATc3 has specifically been implicated in vasculature development, regulation of smooth muscle contractile phenotype, and modulation of vascular smooth muscle contractility [PMID:11439183,17148444]. The calculated molecular weight of NFATC3 is 115 kDa, but the post-modified protein is about 130-170 kDa (PMID: 15728531, PMID: 15857835)

实验方案

Product Specific Protocols
WB protocol for NFATC3 antibody 18222-1-APDownload protocol
IHC protocol for NFATC3 antibody 18222-1-APDownload protocol
IP protocol for NFATC3 antibody 18222-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ChIP

Immunity

Metformin inhibition of mitochondrial ATP and DNA synthesis abrogates NLRP3 inflammasome activation and pulmonary inflammation.

Authors - Hongxu Xian
humanWB

Gastroenterology

Regulator of Calcineurin 1 Gene Isoform 4, Downregulated in Hepatocellular Carcinoma, Prevents Proliferation, Migration, and Invasive Activity of Cancer Cells and Growth of Orthotopic Tumors by Inhibiting Nuclear Translocation of NFAT1.

Authors - Haojie Jin
mouseWB,IP

Cell Death Differ

Trim39 regulates neuronal apoptosis by acting as a SUMO-targeted E3 ubiquitin-ligase for the transcription factor NFATc3.

Authors - Meenakshi Basu-Shrivastava
humanWB

Oncogene

Regulator of calcineurin 1 gene isoform 4 in pancreatic ductal adenocarcinoma regulates the progression of tumor cells.

Authors - Mengyi Lao
humanWB

Cancer Lett

TRPC3 promotes tumorigenesis of gastric cancer via the CNB2/GSK3β/NFATc2 signaling pathway.

Authors - Da-Cen Lin
humanWB

Cell Biol Toxicol

HNRNPA2B1 stabilizes NFATC3 levels to potentiate its combined actions with FOSL1 to mediate vasculogenic mimicry in GBM cells

Authors - Hanting Wang
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