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NFATC3 Polyclonal antibody

NFATC3 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

IHC, IP, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 18222-1-AP

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Synonyms

NF AT4, NF ATc3, NFAT4, NFATC3, NFATX



经过测试的应用

Positive WB detected inJurkat cells, K-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman ovary tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy NFATC3 Ready-To-Use IHC Kit. NFATC3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18222-1-AP targets NFATC3 in WB, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IP, WB, ELISA
Cited Applications chIP, IF, IHC, IP, WB
Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Immunogen NFATC3 fusion protein Ag12985 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3
Synonyms NF AT4, NF ATc3, NFAT4, NFATC3, NFATX
Calculated Molecular Weight 116 kDa
Observed Molecular Weight 130-170 kDa
GenBank Accession NumberBC001050
Gene Symbol NFATC3
Gene ID (NCBI) 4775
RRIDAB_2152773
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12968
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NFAT (nuclear factors of activated T cells) proteins are a family of transcription factors originally identified as mediators of activation of cytokine genes in response to antigenic stimulation of T cells. NFAT proteins also play varied roles in cells outside of the immune system [PMID:11877454]. NFATc3 has specifically been implicated in vasculature development, regulation of smooth muscle contractile phenotype, and modulation of vascular smooth muscle contractility [PMID:11439183,17148444]. The calculated molecular weight of NFATC3 is 115 kDa, but the post-modified protein is about 130-170 kDa (PMID: 15728531, PMID: 15857835)

实验方案

Product Specific Protocols
WB protocol for NFATC3 antibody 18222-1-APDownload protocol
IHC protocol for NFATC3 antibody 18222-1-APDownload protocol
IP protocol for NFATC3 antibody 18222-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ChIP

Immunity

Metformin inhibition of mitochondrial ATP and DNA synthesis abrogates NLRP3 inflammasome activation and pulmonary inflammation.

Authors - Hongxu Xian
humanWB

Gastroenterology

Regulator of Calcineurin 1 Gene Isoform 4, Downregulated in Hepatocellular Carcinoma, Prevents Proliferation, Migration, and Invasive Activity of Cancer Cells and Growth of Orthotopic Tumors by Inhibiting Nuclear Translocation of NFAT1.

Authors - Haojie Jin
mouseWB,IP

Cell Death Differ

Trim39 regulates neuronal apoptosis by acting as a SUMO-targeted E3 ubiquitin-ligase for the transcription factor NFATc3.

Authors - Meenakshi Basu-Shrivastava
humanWB

Oncogene

Regulator of calcineurin 1 gene isoform 4 in pancreatic ductal adenocarcinoma regulates the progression of tumor cells.

Authors - Mengyi Lao
humanWB

Cancer Lett

TRPC3 promotes tumorigenesis of gastric cancer via the CNB2/GSK3β/NFATc2 signaling pathway.

Authors - Da-Cen Lin
humanIF

Cells

Blocking Store-Operated Ca2+ Entry to Protect HL-1 Cardiomyocytes from Epirubicin-Induced Cardiotoxicity

Authors - Xian Liu