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验证数据展示

  • IF Staining of HepG2 using CL488-66924

    IF Staining of HepG2 using CL488-66924

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 NFATC3 antibody (CL488-66924, Clone: 1C1H8 ) at dilution of 1:200, CL594-Phalloidin (red).

CoraLite® Plus 488-conjugated NFATC3 Monoclonal antibody

NFATC3 Monoclonal Antibody for IF/ICC
Cat No. CL488-66924

Print datasheet

CloneNo. 1C1H8

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Synonyms

NF-AT4c, NF-AT4, NFAT4, NF ATc3, NF AT4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  {{ptg:cur_Conjugation}}
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy NFATC3 Ready-To-Use IHC Kit. NFATC3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66924-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66924 targets NFATC3 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen NFATC3 fusion protein Ag27306 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3
Synonyms NF-AT4c, NF-AT4, NFAT4, NF ATc3, NF AT4
Calculated Molecular Weight 116 kDa
Observed Molecular Weight 160 kDa
GenBank Accession NumberBC001050
Gene Symbol NFATC3
Gene ID (NCBI) 4775
RRIDAB_2934505
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ12968
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NFAT (nuclear factors of activated T cells) proteins are a family of transcription factors originally identified as mediators of activation of cytokine genes in response to antigenic stimulation of T cells. NFAT proteins also play varied roles in cells outside of the immune system [PMID:11877454]. NFATc3 has specifically been implicated in vasculature development, regulation of smooth muscle contractile phenotype, and modulation of vascular smooth muscle contractility [PMID:11439183,17148444]. The calculated molecular weight of NFATC3 is 115 kDa, but the post-modified protein is about 130-170 kDa (PMID: 15728531, PMID: 15857835)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NFATC3 antibody CL488-66924Download protocol
Standard Protocols
Click here to view our Standard Protocols