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验证数据展示

  • WB analysis using 26311-1-AP

    WB analysis using 26311-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26311-1-AP (NFATC4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 26311-1-AP

    IHC staining of human lung cancer using 26311-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26311-1-AP (NFATC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NFATC4 antibody included
  • IHC staining of human lung cancer using 26311-1-AP

    IHC staining of human lung cancer using 26311-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26311-1-AP (NFATC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NFATC4 antibody included

NFATC4 Polyclonal antibody

NFATC4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26311-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

NF AT3, NF ATc4, NFAT3, NFATC4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, MCF-7 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy NFATC4 Ready-To-Use IHC Kit. NFATC4 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26311-1-AP targets NFATC4 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 NFATC4 fusion protein Ag24503 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4
别名 NF AT3, NF ATc4, NFAT3, NFATC4
计算分子量 95 kDa
观测分子量 90-105 kDa
GenBank蛋白编号BC053855
基因名称 NFATC4
Gene ID (NCBI) 4776
RRIDAB_3085854
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14934
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NFATC4, also named NFAT3, is a member from the Nuclear Factor of Activated T cells (NFATs) transcription factor family. NFATc4 contains a conserved Rel homology domain (RHD) which includes the DNA-binding motif, and two transactivation domains (TADs) which locate at the N-terminus and C-terminus respectively. NFATC4 is involved in several physiological processes, including the development and function of the immune, cardiovascular, musculoskeletal, and nervous systems. It is also reported that NFATC4 could take part in cell growth, cell migration, tumor metastasis, and patient survival recently.

实验方案

Product Specific Protocols
WB protocol for NFATC4 antibody 26311-1-APDownload protocol
IHC protocol for NFATC4 antibody 26311-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols