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NFE2 Polyclonal antibody

NFE2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 11089-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (2)

p45 NF E2, p45, Nuclear factor, erythroid-derived 2 45 kDa subunit, NF E2, Leucine zipper protein NF-E2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inK-562 cells, U-937 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy NFE2 Ready-To-Use IHC Kit. NFE2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11089-1-AP targets NFE2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, CoIP
经测试反应性 human
文献引用反应性human, mouse
免疫原 NFE2 fusion protein Ag1570 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nuclear factor (erythroid-derived 2), 45kDa
别名 p45 NF E2, p45, Nuclear factor, erythroid-derived 2 45 kDa subunit, NF E2, Leucine zipper protein NF-E2
计算分子量 41 kDa
观测分子量 45 kDa
GenBank蛋白编号BC005044
基因名称 NFE2
Gene ID (NCBI) 4778
RRIDAB_2152928
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16621
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The transcription factor p45 nuclear factor-erythroid-derived 2(NFE2) is one component of the NF-E2 complex that essential for regulating reythroid and megakaryocytic maturation and differentiation. It also plays crucial roles in erythroid and megakaryocytic lineages. NFE2 recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters, and acts as a activator. And it may has a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron. This is a rabbit polyclonal antibody raised against full-length NFE2 of human origin.

实验方案

Product Specific Protocols
WB protocol for NFE2 antibody 11089-1-APDownload protocol
IHC protocol for NFE2 antibody 11089-1-APDownload protocol
IF protocol for NFE2 antibody 11089-1-APDownload protocol
IP protocol for NFE2 antibody 11089-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Am Coll Cardiol

Heme levels are increased in human failing hearts.

Authors - Khechaduri Arineh A
mouse,human

Cell Rep

Targeting CXCR1 alleviates hyperoxia-induced lung injury through promoting glutamine metabolism

Authors - Hao Qin
human,mouseWB,CoIP

Life Sci

Loss of NF-E2 expression contributes to the induction of profibrotic signaling in diabetic kidneys.

Authors - Shunying Jin
  • KD Validated
humanWB

Life Sci

ERK and p38 MAPK inhibition controls NF-E2 degradation and profibrotic signaling in renal proximal tubule cells.

Authors - Jia Li
humanWB

BMC Genomics

Paired guide RNA CRISPR-Cas9 screening for protein-coding genes and lncRNAs involved in transdifferentiation of human B-cells to macrophages.

Authors - Carme Arnan
mouseWB

Adv Sci (Weinh)

Fine-Tuning of Cholesterol Homeostasis Controls Erythroid Differentiation.

Authors - Zhiyuan Lu
  • KD Validated
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