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验证数据展示

  • WB analysis of HL-60 using 66436-1-Ig

    WB analysis of HL-60 using 66436-1-Ig

    HL-60 cells were subjected to SDS PAGE followed by western blot with 66436-1-Ig (NFE2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

NFE2 Monoclonal antibody

NFE2 Monoclonal Antibody for WB, ELISA
Cat No. 66436-1-Ig

Print datasheet

CloneNo. 1D7C8

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Synonyms

p45, Nuclear factor, erythroid-derived 2 45 kDa subunit, NF E2, Leucine zipper protein NF-E2, Leucine zipper protein NF E2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHL-60 cells, K-562 cells
Planning an IHC experiment? We recommend our IHCeasy NFE2 Ready-To-Use IHC Kit. NFE2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66436-1-Ig targets NFE2 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen NFE2 fusion protein Ag1570 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name nuclear factor (erythroid-derived 2), 45kDa
Synonyms p45, Nuclear factor, erythroid-derived 2 45 kDa subunit, NF E2, Leucine zipper protein NF-E2, Leucine zipper protein NF E2
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC005044
Gene Symbol NFE2
Gene ID (NCBI) 4778
RRIDAB_2881806
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16621
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The transcription factor p45 nuclear factor-erythroid-derived 2(NFE2) is one component of the NF-E2 complex that essential for regulating reythroid and megakaryocytic maturation and differentiation. It also plays crucial roles in erythroid and megakaryocytic lineages. NFE2 recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters, and acts as a activator. And it may has a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.

实验方案

Product Specific Protocols
WB protocol for NFE2 antibody 66436-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols