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- KD/KO Validated
NFE2L1 Polyclonal antibody
NFE2L1 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse skeletal muscle tissue, Fetal mouse bone tissue, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells |
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human breast cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12936-1-AP targets NFE2L1 in WB, IHC, IF, IP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB, IF, IP, chIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | NFE2L1 fusion protein Ag3916 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | nuclear factor (erythroid-derived 2)-like 1 |
| Synonyms | HBZ17, LCR F1, Locus control region factor 1, NF E2 related factor 1, NFE2 related factor 1, NFE2L1, NRF1, TCF 11, TCF11, Transcription factor 11, Transcription factor HBZ17, Transcription factor LCR F1 |
| Calculated Molecular Weight | 772 aa, 85 kDa |
| Observed Molecular Weight | 65 kDa |
| GenBank Accession Number | BC010623 |
| Gene Symbol | NFE2L1 |
| Gene ID (NCBI) | 4779 |
| RRID | AB_2267298 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14494 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NFE2L1, also named as HBZ17, NRF1, TCF11 and LCR-F1, belongs to the bZIP family and CNC subfamily. It activates erythroid-specific, globin gene expression. NFE2L1 is involved in mediating ascorbic acid-induced osterix expression and osteoblast differentiation via binding to the antioxidant response element of the osterix promoter. NFE2L1, produced by osteoblasts, is involved in regulating osterix expression, osteoblast differentiation, and bone formation. (PMID:19887580). This antibody is a rabbit polyclonal antibody raised against a region of human NFE2L1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NFE2L1 antibody 12936-1-AP | Download protocol |
| IHC protocol for NFE2L1 antibody 12936-1-AP | Download protocol |
| IP protocol for NFE2L1 antibody 12936-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Transl Med PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma. | ||
Redox Biol Long isoforms of NRF1 negatively regulate adipogenesis via suppression of PPARγ expression. | ||
Free Radic Biol Med Novel mechanistic insight on the neuroprotective effect of berberine: The role of PPARδ for antioxidant action. | ||
Ecotoxicol Environ Saf Long-isoform NFE2L1 silencing inhibits acquisition of malignant phenotypes induced by arsenite in human bronchial epithelial cells.
| ||
Food Chem Toxicol Nfe2l1 deficiency mitigates streptozotocin-induced pancreatic β-cell destruction and development of diabetes in male mice |
