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NFE2L3 Polyclonal antibody

NFE2L3 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 17745-1-AP

Print datasheet

Synonyms

NF E2 related factor 3, NFE2 related factor 3, NFE2L3, NRF3

验证数据展示

  • WB analysis of human placenta using 17745-1-AP

    WB analysis of human placenta using 17745-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 17745-1-AP (NFE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 17745-1-AP

    IF Staining of HepG2 using 17745-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 17745-1-AP (NFE2L3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inhuman placenta tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17745-1-AP targets NFE2L3 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen NFE2L3 fusion protein Ag12005 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nuclear factor (erythroid-derived 2)-like 3
Synonyms NF E2 related factor 3, NFE2 related factor 3, NFE2L3, NRF3
Calculated Molecular Weight 694 aa, 76 kDa
Observed Molecular Weight 76 kDa
GenBank Accession NumberBC068455
Gene Symbol NFE2L3
Gene ID (NCBI) 9603
RRIDAB_2153211
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y4A8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NFE2L3 antibody 17745-1-APDownload protocol
IF protocol for NFE2L3 antibody 17745-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Comput Methods Programs Biomed

Transcription factor NFE2L3 promotes the proliferation of esophageal squamous cell carcinoma cells and causes radiotherapy resistance by regulating IL-6

Authors - Tingting Chen
  • KD Validated
View Article
humanWB

J Oncol

miR-23b-3p Inhibits the Oncogenicity of Colon Adenocarcinoma by Directly Targeting NFE2L3.

Authors - Guohong Huang
View Article