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验证数据展示

  • WB analysis using 66999-1-Ig (same clone as 66999-1-PBS)

    WB analysis using 66999-1-Ig (same clone as 66999-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66999-1-Ig (NFIA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66999-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 66999-1-Ig (same clone as 66999-1-PBS)

    IP experiment of HeLa using 66999-1-Ig (same clone as 66999-1-PBS)

    IP result of anti-NFIA (IP:66999-1-Ig, 5ug; Detection:66999-1-Ig 1:500) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 66999-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 66999-1-Ig (same clone as 66999-1-PBS)

    WB analysis of MCF-7 using 66999-1-Ig (same clone as 66999-1-PBS)

    WB result of NFIA antibody (66999-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NFIA transfected MCF-7 cells. This data was developed using the same antibody clone with 66999-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

NFIA Monoclonal antibody, PBS Only

NFIA Monoclonal Antibody for WB, IP, Indirect ELISA
Cat No. 66999-1-PBS

产品说明书

CloneNo. 3A6D7

宿主/亚型

Mouse / IgG1

种属反应性

Human, rat, mouse, pig, rabbit

应用

WB, IP, Indirect ELISA

别名

CTF, DKFZp434L0422, DKFZp686J23256, FLJ39164, KIAA1439, NF I/A, NF1 A, NFI A, NFI L, NFIA, Nuclear factor 1 A type, Nuclear factor 1/A, nuclear factor I/A, TGGCA binding protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy NFIA Ready-To-Use IHC Kit. NFIA primary antibody included.

产品信息

66999-1-PBS targets NFIA in WB, IP, Indirect ELISA applications and shows reactivity with Human, rat, mouse, pig, rabbit samples.

经测试应用 WB, IP, Indirect ELISA Application Description
经测试反应性 Human, rat, mouse, pig, rabbit
免疫原 NFIA fusion protein Ag17873 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 nuclear factor I/A
别名 CTF, DKFZp434L0422, DKFZp686J23256, FLJ39164, KIAA1439, NF I/A, NF1 A, NFI A, NFI L, NFIA, Nuclear factor 1 A type, Nuclear factor 1/A, nuclear factor I/A, TGGCA binding protein
计算分子量 498 aa, 55 kDa
观测分子量 60-70 kDa
GenBank蛋白编号BC022264
基因名称 NFIA
Gene ID (NCBI) 4774
RRIDAB_2882316
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ12857
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The NFI (nuclear factor I) family consists of four members in vertebrates (NFI-A, NFI-B, NFI-C and NFI-X), and the four NFI genes are expressed in unique patterns during mouse embryogenesis and in the adult. Four isoforms of NFIA were found in human and they play various roles in DNA replication, DNA-dependent transcritpion via their DNA binding property. Multiple residues of NFIA can be phosphorylated resulting in mild shifts of its practical molecular weight. Recent finding also revealed its neuroprotective function in NMDA-induced neuronal damage. The calculated molecular weight of NFIA is 55 kDa, However the size of the proteins cross-linked to the adenoviral NF-I element ranged from 60 to 80 kDa . The larger size observed by us could be due to the oligo protein complex, which would increase the size by 15-20 kDa. (PMID: 11447215)