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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated NFIA Monoclonal antibody

NFIA Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse, pig, rabbit

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3A6D7

Cat No : CL488-66999

Print datasheet

Synonyms

CTF, DKFZp434L0422, DKFZp686J23256, FLJ39164, KIAA1439, NF I/A, NF1 A, NFI A, NFI L, NFIA, Nuclear factor 1 A type, Nuclear factor 1/A, nuclear factor I/A, TGGCA binding protein

验证数据展示

Planning an IHC experiment? We recommend our IHCeasy NFIA Ready-To-Use IHC Kit. NFIA primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66999-1-Ig

产品信息

CL488-66999 targets NFIA in FC (Intra) applications and shows reactivity with Human, rat, mouse, pig, rabbit samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Human, rat, mouse, pig, rabbit
Immunogen NFIA fusion protein Ag17873 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name nuclear factor I/A
Synonyms CTF, DKFZp434L0422, DKFZp686J23256, FLJ39164, KIAA1439, NF I/A, NF1 A, NFI A, NFI L, NFIA, Nuclear factor 1 A type, Nuclear factor 1/A, nuclear factor I/A, TGGCA binding protein
Calculated Molecular Weight 498 aa, 55 kDa
Observed Molecular Weight 60-70 kDa
GenBank Accession NumberBC022264
Gene Symbol NFIA
Gene ID (NCBI) 4774
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ12857
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The NFI (nuclear factor I) family consists of four members in vertebrates (NFI-A, NFI-B, NFI-C and NFI-X), and the four NFI genes are expressed in unique patterns during mouse embryogenesis and in the adult. Four isoforms of NFIA were found in human and they play various roles in DNA replication, DNA-dependent transcritpion via their DNA binding property. Multiple residues of NFIA can be phosphorylated resulting in mild shifts of its practical molecular weight. Recent finding also revealed its neuroprotective function in NMDA-induced neuronal damage. The calculated molecular weight of NFIA is 55 kDa, However the size of the proteins cross-linked to the adenoviral NF-I element ranged from 60 to 80 kDa . The larger size observed by us could be due to the oligo protein complex, which would increase the size by 15-20 kDa. (PMID: 11447215)

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 NFIA antibody CL488-66999Download protocol
Standard Protocols
Click here to view our Standard Protocols