验证数据展示
经过测试的应用
Positive WB detected in | HUVEC cells, HeLa cells, HepG2 cells, NIH/3T3 cells |
Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells, HeLa cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
29898-1-AP targets NFIB in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
Tested Reactivity | human, mouse |
Immunogen | NFIB fusion protein Ag31946 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | nuclear factor I/B |
Synonyms | NF1-B, NF1 B, NF I/B, HMGIC/NFIB, CTF |
Calculated Molecular Weight | 47 kDa |
Observed Molecular Weight | 45-65 kDa |
GenBank Accession Number | BC001283 |
Gene Symbol | NFIB |
Gene ID (NCBI) | 4781 |
RRID | AB_3086183 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O00712 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NFIB, also named as Nuclear factor 1, is a 420 amino acid protein, which belongs to the CTF/NF-I family and may bind DNA as a homodimer. NFIB recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. NFIB also exists in multiple isoforms in prostate cancer cells, with average molecular weights of 62 kDa, 57 kDa, and 49 kDa (PMID: 32692871).
实验方案
Product Specific Protocols | |
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WB protocol for NFIB antibody 29898-1-AP | Download protocol |
IHC protocol for NFIB antibody 29898-1-AP | Download protocol |
IF protocol for NFIB antibody 29898-1-AP | Download protocol |
FC protocol for NFIB antibody 29898-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |