NFKB2,p100 Recombinant antibody

NFKB2,p100 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84022-5-RR

产品说明书

CloneNo. 241126C9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

NFKB2, LYT10, LYT 10, H2TF1, DNA-binding factor KBF2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, Jurkat cells, SKOV-3 cells, HL-60 cells
Positive FC (Intra) detected inA431 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy NFKB2 Ready-To-Use IHC Kit. NFKB2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84022-5-RR targets NFKB2,p100 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 NFKB2,p100 fusion protein Ag0662 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
别名 NFKB2, LYT10, LYT 10, H2TF1, DNA-binding factor KBF2
计算分子量 97 kDa
观测分子量 100-110 kDa
GenBank蛋白编号BC002844
基因名称 NFKB2
Gene ID (NCBI) 4791
ENSEMBL Gene IDENSG00000077150
RRIDAB_3671590
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ00653
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. P52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. P52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. This antibody can bind p100 isoform of NFKB2 spefically, but not p52 isoform.

实验方案

Product Specific Protocols
WB protocol for NFKB2,p100 antibody 84022-5-RRDownload protocol
FC protocol for NFKB2,p100 antibody 84022-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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