验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, K-562 cells, Jurkat cells, SKOV-3 cells, HL-60 cells |
Positive FC (Intra) detected in | A431 cells, HeLa cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
84022-5-RR targets NFKB2,p100 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.
Tested Applications | WB, FC (Intra), ELISA Application Description |
Tested Reactivity | human |
Immunogen | NFKB2,p100 fusion protein Ag0662 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100) |
Synonyms | NFKB2, LYT10, LYT 10, H2TF1, DNA-binding factor KBF2 |
Calculated Molecular Weight | 97 kDa |
Observed Molecular Weight | 100-110 kDa |
GenBank Accession Number | BC002844 |
Gene Symbol | NFKB2 |
Gene ID (NCBI) | 4791 |
ENSEMBL Gene ID | ENSG00000077150 |
RRID | AB_3671590 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | Q00653 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. P52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. P52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. This antibody can bind p100 isoform of NFKB2 spefically, but not p52 isoform.
实验方案
Product Specific Protocols | |
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WB protocol for NFKB2,p100 antibody 84022-5-RR | Download protocol |
FC protocol for NFKB2,p100 antibody 84022-5-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |