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验证数据展示

  • WB analysis using 12660-1-AP

    WB analysis using 12660-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12660-1-AP (IkB beta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 12660-1-AP

    IHC staining of human colon using 12660-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12660-1-AP (IkB beta antibody at dilution of 1:200 (under 10x lens).

  • IHC staining of human colon using 12660-1-AP

    IHC staining of human colon using 12660-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12660-1-AP (IkB beta antibody at dilution of 1:200 (under 40x lens).

  • IF Staining of HeLa using 12660-1-AP

    IF Staining of HeLa using 12660-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using IkB Beta antibody (12660-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

IkB Beta Polyclonal antibody

IkB Beta Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12660-1-AP

Print datasheet

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

IκB beta, IκB β, NFKBIB, IkB-B, IKBB

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, MCF-7 cells
Positive IHC detected inhuman colon tissue, human endometrial cancer tissue, human colon cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

12660-1-AP targets IkB Beta in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen IkB Beta fusion protein Ag3340 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta
Synonyms IκB beta, IκB β, NFKBIB, IkB-B, IKBB
Calculated Molecular Weight 356 aa, 38 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC015528
Gene Symbol IkB Beta
Gene ID (NCBI) 4793
RRIDAB_2151611
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15653
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for IkB Beta antibody 12660-1-APDownload protocol
IHC protocol for IkB Beta antibody 12660-1-APDownload protocol
IF protocol for IkB Beta antibody 12660-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Toxicol Appl Pharmacol

Salinomycin promotes T-cell proliferation by inhibiting the expression and enzymatic activity of immunosuppressive indoleamine-2,3-dioxygenase in human breast cancer cells.

Authors - Azubuike Peter Ebokaiwe
View Article
mouseWB

Gut Microbes

Gut microbiota regulates host melatonin production through epithelial cell MyD88

Authors - Bingnan Liu
View Article