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验证数据展示

  • WB analysis using 12660-1-AP

    WB analysis using 12660-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12660-1-AP (IkB beta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 12660-1-AP

    IHC staining of human colon using 12660-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12660-1-AP (IkB beta antibody at dilution of 1:200 (under 10x lens).

  • IHC staining of human colon using 12660-1-AP

    IHC staining of human colon using 12660-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12660-1-AP (IkB beta antibody at dilution of 1:200 (under 40x lens).

  • IF Staining of HeLa using 12660-1-AP

    IF Staining of HeLa using 12660-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using IkB Beta antibody (12660-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

IkB Beta Polyclonal antibody

IkB Beta Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12660-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, ELISA

别名

IκB beta, IκB β, NFKBIB, IkB-B, IKBB

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, MCF-7 cells
Positive IHC detected inhuman colon tissue, human endometrial cancer tissue, human colon cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

12660-1-AP targets IkB Beta in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human, mouse
免疫原 IkB Beta fusion protein Ag3340 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta
别名 IκB beta, IκB β, NFKBIB, IkB-B, IKBB
计算分子量 356 aa, 38 kDa
观测分子量 46 kDa
GenBank蛋白编号BC015528
基因名称 IkB Beta
Gene ID (NCBI) 4793
RRIDAB_2151611
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15653
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for IkB Beta antibody 12660-1-APDownload protocol
IHC protocol for IkB Beta antibody 12660-1-APDownload protocol
IF protocol for IkB Beta antibody 12660-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Toxicol Appl Pharmacol

Salinomycin promotes T-cell proliferation by inhibiting the expression and enzymatic activity of immunosuppressive indoleamine-2,3-dioxygenase in human breast cancer cells.

Authors - Azubuike Peter Ebokaiwe
View Article
mouseWB

Gut Microbes

Gut microbiota regulates host melatonin production through epithelial cell MyD88

Authors - Bingnan Liu
View Article